Baculovirus-expressed FAdV-4 penton base protein protects chicken against hepatitis-hydropericardium syndrome

ZHANG Jun-qin; WEI Yan-ming; HUANG Kun; SUN Xiao-mei; ZOU Zhong; JIN Mei-lin

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Baculovirus-expressed FAdV-4 penton base protein protects chicken against hepatitis-hydropericardium syndrome

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Hepatitis-hydropericardium syndrome(HHS)is an infectious disease caused by fowl adenovirus serotype 4(FAdV-4).Several structural and non-structural proteins of FAdV-4 have been expressed in Escherichia coli and baculovirus expression system to develop candidate subunit vaccines.However,the protective efficiency of baculovirus-expressed penton base protein has not been assessed.In this study,two recombinant capsid proteins,penton base and fiber-2,were constructed.And then,penton base and fiber-2 were administrated alone or together to specific pathogen-free(SPF)chickens at 14 days of life and boosted at 28 days of life.At 42 days of life,the immunized groups and the control group were challenged with FAdV-4 virulent strain.Results show that inoculating penton base or penton base+fiber-2 provided 100%protection to the chickens.All groups vaccinated with the recombinant protein produced detectable antibodies and showed no apparent lesions.Thus,baculovirus-expressed penton base protein is a promising candidate subunit vaccine.

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《农业科学学报（英文版）》 |2019年第11期|2598-2604|共7页
作者
ZHANG Jun-qin; WEI Yan-ming; HUANG Kun; SUN Xiao-mei; ZOU Zhong; JIN Mei-lin;
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State Key Laboratory of Agricultural Microbiology Huazhong Agricultural University Wuhan 430070 P.R.China;

College of Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P.R.China;

Key Laboratory of Development of Veterinary Diagnostic Products Ministry of Agriculture Wuhan 430070 P.R.China;

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Baculovirus-expressed FAdV-4 penton base protein protects chicken against hepatitis-hydropericardium syndrome

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