As one member of the Ras super family,Rheb is an upstream regulator of mTOR signaling pathway,which regulates the process of cell-growth,proliferation and differentiation.In order to study the relationship between Rheb and mTOR in Inner Mongolian Cashmere goat(Capra hircus) cells,Ras homolog enriched in brain(Rheb) gene cDNA was amplified by RT-PCR.It is 555 bp in length and includes the complete ORF encoding 184 amino acids(GenBank accession no.HM569224).The full cDNA nucleotide sequence has a99% identity with that of sheep,98% with cattle and 93% with human while their amino acids sequence shares identity with 98,97 and 97% of them,correspondingly.The bioinformatics analysis showed that Rheb has a Ras family domain,two casein kinase II phosphorylation sites,two ATP/GTP-binding sites motif A(P-loop),a prenyl group binding site(CAAX box).Tissue-specific expression analysis performed by semi-quantitative RT-PCR.The Rheb gene was expressed in all the tested tissues and the highest level of mRNA accumulation was detected in brain,suggesting that Rheb played an important role in goat cells.
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