Functional Characterization of a NEM1-Like Gene in Magnaporthe oryzae

WANG Ying; JIAO Tian-lei; LIU Xiao-hong; LIN Fu-chen; WU Wei-ren

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Functional Characterization of a NEM1-Like Gene in Magnaporthe oryzae

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Magnaporthe oryzae,a filamentous ascomycete fungus,is well known as the causal agent of rice blast.With the technology of suppression subtractive hybridization(SSH),it was previously found that MGG_06001(or named MoNEM1),a gene of M.oryzae homologous to the NEM1(nuclear envelope morphology protein 1) gene of baker's yeast(Saccharomyces cerevisiae),is differentially expressed between the mature appressium and the conidium and mycelium.This study aimed to characterize the function of MoNEM1 gene by knocking it out using the method of target gene replacement.The DMonem1 mutants exhibited reduced mycelial growth and conidiation.However,disruption of MoNEM1 gene does not affect the pathogenicity of M.oryzae on barley and rice.

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《农业科学学报（英文版）》 |2011年第9期|1385-1390|共6页
作者
WANG Ying; JIAO Tian-lei; LIU Xiao-hong; LIN Fu-chen; WU Wei-ren;
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Department of Agronomy College of Agriculture & Biotechnology Zhejiang University Hangzhou 310029 P.R.China;

Institute of Biotechnology College of Agriculture & Biotechnology Zhejiang University Hangzhou 310029 P.R.China;

School of Life Sciences Fujian Agriculture & Forestry University Fuzhou 350002 P.R. China;

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Functional Characterization of a NEM1-Like Gene in Magnaporthe oryzae

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