Real-time PCR assay for detection of Dickeya fangzhongdai causing bleeding canker of pear disease in China

TIAN Yan-li; ZHAO Yu-qiang; CHEN Bao-hui; CHEN Shuo; ZENG Rong; HU Bai-shi; LI Xiang

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Real-time PCR assay for detection of Dickeya fangzhongdai causing bleeding canker of pear disease in China

机译：实时荧光定量PCR检测中国梨病的Dickeya fangzhongdai致出血性溃疡

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Bleeding canker, caused by Dickeya fangzhongdai, is a devastating disease of pear in China. The bacterium causes cankers, branch die-back, and eventually kills pear trees. The typical sign of bleeding canker infection is a rusty-brown bacterial ooze that exudes down from cankers onto branches or trunks. However, early symptoms and signs are inconspicuous, which makes effective disease management difficult. Detection and identification of D. fangzhongdai are time-consuming and difficult because no rapid method exists to date. In this study, a Taq Man real-time PCR assay was developed for D. fangzhongdai based on an elongation factor G(fus A) gene. The real-time PCR assay detected 0.2 pg μL^-1 DNA and 1×10^3 Cfu m L^-1 of D. fangzhongdai. Based on this assay, bleeding canker on asymptomatic pear trees can be diagnosed as early as 5 days after infection. The real-time PCR assay can facilitate disease management by providing early and accurate diagnosis of the bleeding canker disease of pear.

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《农业科学学报（英文版）》 |2020年第4期|898-905|共8页
作者
TIAN Yan-li; ZHAO Yu-qiang; CHEN Bao-hui; CHEN Shuo; ZENG Rong; HU Bai-shi; LI Xiang;
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作者单位

College of Plant Protection and Key Laboratory of Integrated Management of Crop Diseases and Pests Ministry of Education Nanjing Agricultural University Nanjing 210095 P.R.China;

Institute of Botany Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen)/Jiangsu Key Laboratory for the Research and Utilization of Plant Resources Nanjing 210014 P.R.China;

Charlottetown Laboratory Canadian Food Inspection Agency Charlottetown PE C1A5T1 Canada;

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Real-time PCR assay for detection of Dickeya fangzhongdai causing bleeding canker of pear disease in China

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