Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda

JlN Ming-hui; TAO Jia-hui; Ll Qi; CHENG Ying; SUN Xiao-xu; WU Kong-ming; XlAO Yu-tao

首页> 中文期刊> 《农业科学学报（英文版）》 >Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda

Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda

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ATP-binding cassette transporter C2(ABCC2)is known to be a receptor for Bacillus thuringiensis(Bt)toxins in several lepidopteran insects.Mutations in the ABCC2 gene have been genetically linked to field-evolved resistance to the Cry1 F toxin from Bt in Spodoptera frugiperda.Here we generated a SfABCC2 knockout strain of S.frugiperda using the CRISPR/Cas9 system to provide further functional evidence of the role of this gene in susceptibility and resistance to Cry1 F.Results from bioassays showed that the SfABCC2 knockout S.frugiperda strain displayed 118-fold resistance to Cry1 F compared with the parental DH19 strain,but no resistance to Vip3 A toxin from Bt.These results provide the first reverse genetic evidence for SfABCC2 as a functional receptor for Cry1 F.

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《农业科学学报（英文版）》 |2021年第3期|815-820|共6页
作者
JlN Ming-hui; TAO Jia-hui; Ll Qi; CHENG Ying; SUN Xiao-xu; WU Kong-ming; XlAO Yu-tao;
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Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518120 P.R.China;

State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 P.R.China;

Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518120 P.R.China;

Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518120 P.R.China;

Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518120 P.R.China;

State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 P.R.China;

State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 P.R.China;

Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518120 P.R.China;

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Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda

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