Analysis of DNA methylation of CD79B in MDV-infected chicken spleen

WANG Lu-lu; ZHAO Chun-fang; LIU Chang-jun; ZHANG Hao; LIAN Ling

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Analysis of DNA methylation of CD79B in MDV-infected chicken spleen

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Marek’s disease(MD),an immunosuppressive disease induced by Marek’s disease virus(MDV),provides an ideal model for studying diseases caused by a carcinogenic virus.CD79 B is a B-cell antigen receptor complex-associated protein β-chain precursor which is involved in the activation,proliferation,differentiation of B-cell and the transmission of downstream signals.This study analyzed CD79 B gene mRNA expression and methylation by two schemes#20(5′flanking to intron 1)and#27(intron 2 to intron 3),between MDV-infected tumorous spleens(TS)and non-infected spleens(NS).Results showed that average methylation levels of CpGs in #20 and #27 were higher in TS than in NS(P<0.05),while,CD79 B mRNA expression was lower in TS than in NS(P<0.01).Six of 40 CpG sites showed significantly(P<0.05)different methylation levels between TS and NS.Correlation analysis showed that the average methylation level rather than a single site methylation level in #20 affected(P<0.05)mRNA expression.Collectively,it was found that the change of CD79 B gene expression after MDV infection might be partly explained by modification of DNA methylation.

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《农业科学学报（英文版）》 |2021年第11期|2995-3002|共8页
作者
WANG Lu-lu; ZHAO Chun-fang; LIU Chang-jun; ZHANG Hao; LIAN Ling;
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National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding College of Animal Science and Technology China Agricultural University Beijing 100193 P.R.China;

National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding College of Animal Science and Technology China Agricultural University Beijing 100193 P.R.China;

Division of Avian Infectious Diseases Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 150001 P.R.China;

National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding College of Animal Science and Technology China Agricultural University Beijing 100193 P.R.China;

National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding College of Animal Science and Technology China Agricultural University Beijing 100193 P.R.China;

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Analysis of DNA methylation of CD79B in MDV-infected chicken spleen

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