Identification of proteins associated with Fusarium crown rot resistance in wheat using label-free quantification analysis

JIN Jing-jing; DUAN Shuo-nan; Ql Yong-zhi; ZHEN Wen-chao; MA Jun

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Identification of proteins associated with Fusarium crown rot resistance in wheat using label-free quantification analysis

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Fusarium crown rot(FCR),typically caused by Fusarium pseudograminearum,is a severe soil-borne disease that,in recent years,has become an emerging threat to Chinese wheat crops.For the first time in this study,we investigated and compared the proteomic characteristics of two Chinese wheat varieties(04 Zhong 36 and Xinmai 26)at 24,48,and 72 h post-inoculation using label-free quantitative proteomic analysis.A total of 9234 proteins were successfully quantified,of which 783 were differentially expressed after inoculation.These proteins were mainly involved in metabolic,single-organism,and cellular processes.Thirty-three proteins associated with defense,cell wall formation,photosynthesis,etc.,showed consistently different expression between the two genotypes at multiple time points.In particular,chitinase,which degrades chitin in the fungal cell wall and limits fungal growth,was exclusively and consistently upregulated in 04 Zhong 36 across the three time points.Other proteins such as flavonoid O-methyltransferase,glycosyltransferase,and peroxidase were only upregulated in 04 Zhong 36,and proteins,including the berberine bridge enzyme and rubisco large subunit-binding protein,were specifically downregulated in Xinmai 26.The expression of transcripts encoding eight selected proteins through qRT-PCR analysis supported the proteomic profiles.Overall,the results of this study allow us to understand FCR resistance in wheat at the protein level.Some proteins and their corresponding genes may be useful resources for the genetic improvement of FCR resistance in wheat.

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《农业科学学报：英文版》 |2021年第12期|3209-3221|共13页
作者
JIN Jing-jing; DUAN Shuo-nan; Ql Yong-zhi; ZHEN Wen-chao; MA Jun;
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作者单位

College of Plant Protection;

Hebei Agricultural University;

Baoding 071001;

P.R.China;

State Key Laboratory of North China Crop Improvement and Regulation;

Baoding 071001;

P.R.China;

College of Agronomy and Biotechnology;

China Agricultural University;

Beijing 100193;

P.R.China;

College of Agronomy;

Hebei Agricultural University;

Baoding 071001;

P.R.China;

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原文格式 PDF
正文语种 chi
中图分类禾谷类作物;
关键词
wheat; Fusarium crown rot; proteomic; differential expression; multiple time points;

Identification of proteins associated with Fusarium crown rot resistance in wheat using label-free quantification analysis

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