A domesticated Harbinger transposase forms a complex with HDA6 and promotes histone H3 deacetylation at genes but not TEs in Arabidopsis

Xishi Zhou; Junna He; Christos N.Velanis; Yiwang Zhu; Yuhan He; Kai Tang; Mingku Zhu; Lisa Graser; Erica de Leau; Xingang Wang; Lingrui Zhang; W.Andy Tao; Justin Goodrich; Jian-Kang Zhu; Cui-Jun Zhang

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A domesticated Harbinger transposase forms a complex with HDA6 and promotes histone H3 deacetylation at genes but not TEs in Arabidopsis

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In eukaryotes,histone acetylation is a major modification on histone N-terminal tails that is tightly connected to transcriptional activation.HDA6 is a histone deacetylase involved in the transcriptional regulation of genes and transposable elements(TEs)in Arabidopsis thaliana.HDA6 has been shown to participate in several complexes in plants,including a conserved SIN3 complex.Here,we uncover a novel protein complex containing HDA6,several Harbinger transposon-derived proteins(HHP1,SANT1,SANT2,SANT3,and SANT4),and MBD domain-containing proteins(MBD1,MBD2,and MBD4).We show that mutations of all four SANT genes in the sant-null mutant cause increased expression of the flowering repressors FLC,MAF4,and MAF5,resulting in a late flowering phenotype.Transcriptome deep sequencing reveals that while the SANT proteins and HDA6 regulate the expression of largely overlapping sets of genes,TE silencing is unaffected in sant-null mutants.Our global histone H3 acetylation profiling shows that SANT proteins and HDA6 modulate gene expression through deacetylation.Collectively,our findings suggest that Harbinger transposon-derived SANT domain-containing proteins are required for histone deacetylation and flowering time control in plants.

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《植物学报：英文版》 |2021年第8期|1462-1474|共13页
作者
Xishi Zhou; Junna He; Christos N.Velanis; Yiwang Zhu; Yuhan He; Kai Tang; Mingku Zhu; Lisa Graser; Erica de Leau; Xingang Wang; Lingrui Zhang; W.Andy Tao; Justin Goodrich; Jian-Kang Zhu; Cui-Jun Zhang;
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作者单位

Shenzhen Branch;

Guangdong Laboratory for Lingnan Modern Agriculture;

Genome Analysis Laboratory of the Ministry of Agriculture;

Agricultural Genomics Institute at Shenzhen;

Chinese Academy of Agricultural Sciences;

Shenzhen;

518124 China;

College of Horticulture;

China Agricultural University;

Beijing;

100193 China;

Department of Horticulture and Landscape Architecture;

Purdue University;

West Lafayette;

IN 47907 USA;

Institute of Molecular Plant Science;

School of Biological Sciences;

University of Edinburgh;

Daniel Rutherford Building;

Max Born Crescent;

Edinburgh;

EH93BF United Kingdom;

Shanghai Center for Plant Stress Biology and CAS Center for Excellence in Molecular Plant Sciences;

Chinese Academy of Sciences;

Shanghai;

201602 China;

Institute of Integrative Plant Biology;

School of Life Sciences;

Jiangsu Normal University;

Xuzhou;

221116 China;

University of Applied Sciences Mannheim;

Paul-Wittsack-Str.10;

Mannheim;

68163 Germany;

Department of Biochemistry;

Purdue University;

West Lafayette;

IN 47907 USA;

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原文格式 PDF
正文语种 chi
中图分类植物基因工程;
关键词
deacetylation; flowering; Harbinger; HDA6; histone acetylation; protein complex; SANT;

A domesticated Harbinger transposase forms a complex with HDA6 and promotes histone H3 deacetylation at genes but not TEs in Arabidopsis

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