Objective:Norcantharidin (NCTD) is a new small molecule with anticarcinogen activity, which was extracted from Chinese cantharides. ABT-737 is another small molecule with anticarcinogen activity in lower dose by inhibiting BCL-2. This study was designed to explore the effect of NCTD combined with ABT-737 on the in vitro proliferation and apoptosis of cervical cancer cells. Methods:HeLa and SiHa cell lines were cultured in vitro. NCTD alone or in combination with ABT-737 was used to treat cells. MTT assay was used to determine the viability of cells. The flow cytometry was used to detect apoptosis. Results:NCTD alone could significantly inhibit the proliferation of HeLa and SiHa in a dose-dependent manner. NCTD combined with ABT-737 could significantly enhance the effect of NCTD. The apoptosis rates of HeLa in the ABT-737 (1μmol/L) group and the NCTD (60 μmol/L) group were (31.65±7.75)% and (40.34±14.52)%, while this rate was (63.03±10.90)% in the NCTD combined ABT-737 group. The apoptosis rates of SiHa in the ABT-737 group and the NCTD group were (9.76±0.04)% and (22.04±3.03)%, respectively; while the rate in the NCTD combined ABT-737 group was (58.36±2.31)%. Effect of the combined two drugs on apoptosis was significantly stronger than that of any single drug (P<0.05). Conclusions:NCTD shows the in vitro anti-proliferation effect on the HeLa and SiHa cell lines and the effects of both inhibiting proliferation and inducing apoptosis when combined with ABT-737.%目的：研究去甲斑蝥素（norcantharidin，NCTD）联合ABT-737对宫颈癌细胞增殖及凋亡的影响。方法：采用四甲基偶氮唑盐（MTT）法检测NCTD单药或联合ABT-737对宫颈癌细胞株HeLa、SiHa增殖的影响；采用流式细胞术分析NCTD联合ABT-737对宫颈癌细胞凋亡的影响。结果：NCTD单药能够显著抑制宫颈癌细胞株HeLa、SiHa的增殖，抑制作用呈剂量依赖性。 NCTD与ABT-737联用后抑制作用明显增强。1μmol/L ABT、60μmol/L NCTD及两药联用诱导HeLa细胞的凋亡率分别为（31.65±7.75）%、（40.34±14.52）%和（63.03±10.90）%，诱导SiHa细胞的凋亡率分别为（9.76±0.04）%、（22.04±3.03）%和（58.36±2.31）%。 NCTD、ABT两药联合的促凋亡作用比单药显著增强（P＜0.05）。结论：NCTD单药在体外能够明显抑制宫颈癌细胞HeLa、SiHa的增殖，与ABT-737联用后抑制增殖和促凋亡作用更加显著。
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