拟南芥氨基酸透性酶1（ AAP1，也叫NAT1）是植物中第一个被分离的氨基酸转运蛋白，在拟南芥主根、侧根、根毛及根表皮细胞中均有增加氨基酸含量的作用。本研究利用RT－PCR的方法，从拟南芥中克隆了该基因，测序结果与 GenBank（NM－104616�4）中已发表的拟南芥 AAP1（AtAAP1）基因的同源性高达99�93％；并将AtAAP1基因与pCAMBIA3300构建p3300⁃AAP1植物表达载体，为过表达AtAAP1基因，提高作物氨基酸含量，从而改善作物氮素利用效率提供参考。%Arabidopsis amino acid permease 1 ( AAP1, also called NAT1) is the first separated a⁃mino acid transporter in plants, and AAP1 gene is an important amino acid transporter to increase the content of amino acids in main roots, lateral roots, root hairs and root epidermal cells. In this study, AtAAP1 gene was cloned from Arabidopsis thaliana by RT-PCR. Sequence homology analysis showed that it had high similarity (99�93%) with Arabidopsis thaliana AAP1 that has been submit⁃ted to GenBank ( NM-104616�4). The plant expression vector p3300⁃AAP1 was constructed by gene AtAAP1 and pCAMBIA3300. It is the over expression of AtAAP1, which can increase amino acid content of crops, and thereby provide reference for improving nitrogen use efficiency.
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