The substances which are structurally and functionally irrelevant to bacterial cells can be discharged through multi⁃material resistant efflux pump. Certain organic solvent tolerant bacteria re⁃move intracellular and cytomembrane⁃mounted organic solvent molecules through the efflux pump. Signs of resistance to antibiotics are generally shown by these solvent tolerant bacteria, therefore, by measuring the bacteria cells′resistance to antibiotics, existence of intracellular organic solvent efflux pump can be confirmed. Experiments on MIC ( minimum inhibitory concentration) and tolerance of Bacillus cereus UD-8 to tetracycline, chloramphenicol, ampicillin and lincomycin were taken during the investigation to preliminarily determine mechanism for organic solvent efflux pump of Bacillus ce⁃reus UD-8.%菌体细胞内存在的与细胞结构和功能不相关的物质可以通过多物质抗性泵出系统运输排出细胞外。某些有机溶剂耐受菌可以通过泵出系统将细胞膜上及细胞内的有机溶剂分子泵出到细胞外。这些有机溶剂耐受菌通常表现出对抗生素耐受性的提高，通过测定细菌细胞抗生素耐受性可以测定其细胞内是否存在有机溶剂外排泵。为确定有机溶剂耐受菌株Bacillus cereus UD－8细胞内是否存在有机溶剂外排泵系统，分别研究了Bacillus cereus UD－8的有机溶剂耐受性；四环素、氯霉素、氨苄青霉素、林可霉素的抗药性及4种抗生素对Bacillus cereus UD－8的最小抑菌浓度。初步断定蜡状芽孢杆菌Bacillus cereus UD－8可能具有有机溶剂泵出机制。
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