Objective:Establishing the method of simultaneous determining the content of multi-component of Phellodendron.Method:Using diamonsil C18 chromatographic column.Result:Getting nice linear relation between the peak area and quality concentration( r≥0.9990);sample recovery more than 99.3%;RSD=0.6%~1.2%.? Conclusion:We get a simple, steady and repreatable method, and provide a reliable method to controland evaluate the quality of Phellodendron amurense rupr.%目的:建立同时测定关黄柏中多组分化学成分含量的方法. 方法:采用 Diamonsil C18色谱柱 (4.6mm ×250mm, 5μm),以乙腈—水(1‰乙酸,2mmol醋酸铵)为流动相梯度洗脱,检测波长为280nm,柱温25℃,流速1ml/min. 结果:黄柏碱、药根碱、巴马汀和小檗碱分别在18.75-130.00, 5.00-100.00,20.00-200.00,0.09-1.80μg/ml峰面积和质量浓度线性关系良好(r≥0.999 0),平均加样回收率分别为99.3%, 97.4%,104.7%,98.7%, RSD分别为1.0%, 0.6%,1.2%,1.4%. 结论:含量测定方法简便、稳定、重复性好,为关黄柏药材质量控制和评价提供了可靠的方法.
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