为了评价拟南芥转录因子AtMYB44基因对小麦抗病能力的影响,以小麦幼胚诱导的愈伤组织为转化受体,以磷酸甘露糖异构酶（phosphomannose isomerase,PMI）基因作为选择标记,通过基因枪介导法将带有At-MYB44基因的表达质粒AF234296-44导入小麦品种扬麦158.经过2～3次甘露糖筛选后,获得75株再生苗.通过氯酚红（chlorophenol red,CPR）染色获得40株CPR阳性再生苗,进一步通过标记基因PMI和目的基因AtMYB44的PCR检测,获得22株PCR阳性植株,转化率为0.306%.结果初步表明AtMYB44基因已整合到小麦基因组中,获得了生物安全标记的转AtMYB44基因小麦.%To unravel the role of AtMYB44 gene,a transcription factor in Arabidopsis thaliana,in enhancing transgenic wheat against pathogens,the selectable marker phosphomannose isomerase（PMI） gene and AtMYB44 gene in plasmid AF234296-44 had been transferred into immature embryos of a spring wheat cultivar Yangmai 158 via microprojectile bombardment.75 regenerated plants were obtained by using the selection medium complemented with L-mannose for 2~3 times.40 transgenic plants were positive by chlorophenol red assay,and then 22 positive plants of them were identified by PCR using AtMYB44 and PMI genes in the T0 generation with the efficiency of 0.306%.These results suggest that AtMYB44 gene has been transfered into wheat genome and the transgenic wheat with antibiotic marker genes is brought about.
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