为了建立适用于嗜热链球菌菌株资源多样性调查的菌株分型方法，尝试将1型CRISPR位点间区序列分析用于嗜热链球菌的菌株分型，并与常用ERIC-PCR指纹图谱方法进行了比较。结果表明，1型CRISPR位点间区序列分析可以把30株从三个不同样品中分离的嗜热链球菌分成三种差异明显的类型：不同类型菌株之间没有相同的间区序列；而同一类型菌株之间，间区序列的组成和排列则基本一致，并且上述分型的结果与用ERIC-PCR指纹图谱技术获得的结果完全一致。因此，1型CRISPR位点间区序列分析是嗜热链球菌分型鉴定的可靠方法，并适用于大量菌株的分型鉴定和多样性调查。%In order to establish a strain typing method suitable for investigating the diversity of Streptococcus thermo-philes resources based on spacers of CRISPR 1 locus attempted to analyze the strain typing of S.thermophiles and com-pared with the routine ERIC-PCR finger print spectrum method .The results showed that the spacers CRISPR 1 locus sequence analyses could divide 30 strains isolated from three different samples into three types with obvious differ-ences:there were no similar spacer among different type of strains; and the component and arrangement among the same type of strain were basically consistent , and furthermore the results of the above mentioned typing totally accor-ded with the results obtained by ERIC-PCR finger print spectrum technology .Therefore, analyses on spacers of CRISPR1 locus was a reliable method for typing characterization of strains of S.thermophiles and was especially suit-able for typing classification and diversity investigation at large amount of strains .
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