Objective:To exPlore the migration and invasion ability of gastric cancer cells after silencing IKKα ex-Pression. Methods:We used Western blot to detect the IKKα exPression in different gastric cancer cells,and then transfected IKKα shRNA lentivirus and control lentivirus into MKN - 28 cells exPressing high level of IKKα. Western blot and RT - PCR were Performed to verified the silencing effect. And then we conducted transwell migration and in-vasion assay and the wound - healing assay to deliberate the role of IKKα in GC metastasis. Results:Western blot showed that the exPression level of IKKα in MKN28 cells were higher than other gastric cancer cell lines. After trans-fection of IKKα shRNA,the Protein and mRNA level of IKKα in the MKN28 - LV - shIKKα was significantly de-creased. The migration and invasion ability of MKN28 - LV - shIKKα was significantly decreased comPared with MKN28 - LV - shcontrol. Conclusion:Silencing of IKKα significantly decreased the invasion and migration ability of gastric cancer cells. Targeting IKKα may therefore Provide a Promising theraPeutic strategy for gastric cancer Patients.%目的：研究降低 IKKα的表达对人胃癌细胞迁移和侵袭能力的影响。方法：利用 Western blot 检测胃癌细胞系中 IKKα蛋白的表达水平，选择 IKKα表达较高的细胞系 MKN28，转染下调 IKKα的慢病毒，建立稳定转染细胞株（标记为 MKN28- LV - shcontrol 和 MKN28- LV - shIKKα），并利用 Western blot、RT - PCR 验证病毒转染效果。采用 Transwell 实验和划痕实验检测低表达 IKKα对胃癌细胞迁移和侵袭能力的影响。结果：Western blot 结果显示胃癌细胞系中 MKN28细胞 IKKα蛋白表达水平相对较高，转染慢病毒干扰载体成功后利用 Western blot 及 RT - PCR 实验证实实验组 MKN28- LV - shIKKα较对照组 MKN28- LV - shcontrol 中IKKα蛋白及 mRNA 水平明显降低。利用 Transwell 及划痕实验证实实验组 MKN28- LV - shIKKα较对照组MKN28- LV - shcontrol 细胞迁移及侵袭能力明显降低。结论：IKKα与胃癌的迁移及侵袭密切相关，降低IKKα的表达水平能显著抑制胃癌细胞的迁移和侵袭能力，提示 IKKα在胃癌的转移中可能发挥着重要作用。
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