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首页> 中文期刊> 《分子细胞生物学报：英文版》 >Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly

Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly

         
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摘要

Error-free cell division depends on the accurate assembly of the spindle midzone from dynamic spindle microtubules to ensure chromatid segregation during metaphase-anaphase transition.However,the mechanism underlying the key transition from the mitotic spindle to central spindle before anaphase onset remains elusive.Given the prevalence of chromosome instability phenotype in gastric tumorigenesis,we developed a strategy to model context-dependent cell division using a combination of light sheet microscope and 3D gastric organoids.Light sheet microscopic image analyses of 3D organoids showed that CENP-E inhibited cells undergoing aberrant metaphase-anaphase transition and exhibiting chromosome segregation errors during mitosis.Highresolution real-time imaging analyses of 2D cell culture revealed that CENP-E inhibited cells undergoing central spindle splitting and chromosome instability phenotype.Using biotinylated syntelin as an affinity matrix,we found that CENP-E forms a complex with PRC1 in mitotic cells.Chemical inhibition of CENP-E in metaphase by syntelin prevented accurate central spindle assembly by perturbing temporal assembly of PRC1 to the midzone.Thus,CENP-E-mediated PRC1 assembly to the central spindle constitutes a temporal switch to organize dynamic kinetochore microtubules into stable midzone arrays.These findings reveal a previously uncharacterized role of CENP-E in temporal control of central spindle assembly.Since CENP-E is absent from yeast,we reasoned that metazoans evolved an elaborate central spindle organization machinery to ensure accurate sister chromatid segregation during anaphase and cytokinesis.

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  • 来源
    《分子细胞生物学报：英文版》 |2020年第8期|P.654-665|共12页
  • 作者

    Xu Liu; Leilei Xu; Junying Li; Phil Y.Yao; Wanjuan Wang; Hazrat Ismail; Haowei Wang; Bryce Liao; Zhihong Yang; Tarsha Ward; Ke Ruan; Jianchun Zhang; Quan Wu; Ping He; Xia Ding; Dongmei Wang; Chuanhai Fu; Zhen Dou; Feng Yan; Wenwen Wang; Xing Liu; Xuebiao Yao;

  • 作者单位

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 ChinaDepartment of Physiology Morehouse School of Medicine Atlanta GA 30310 USA;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 China;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 China;

    Department of Physiology Morehouse School of Medicine Atlanta GA 30310 USA;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 ChinaSchool of Basic Medical Sciences Beijing University of Chinese Medicine Beijing 100029 China;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 China;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 China;

    Department of Physiology Morehouse School of Medicine Atlanta GA 30310 USADepartment of Biology Duke University Durham NC 27708 USA;

    Institute of ProteoGenomics Beijing 100029 China;

    Department of Physiology Morehouse School of Medicine Atlanta GA 30310 USAHarvard Medical School Boston MA 02115 USA;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 China;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 China;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 China;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 China;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 ChinaSchool of Basic Medical Sciences Beijing University of Chinese Medicine Beijing 100029 China;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 ChinaDepartment of Physiology Morehouse School of Medicine Atlanta GA 30310 USA;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 China;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 ChinaDepartment of Physiology Morehouse School of Medicine Atlanta GA 30310 USA;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 China;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 ChinaDepartment of Physiology Morehouse School of Medicine Atlanta GA 30310 USA;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 ChinaDepartment of Physiology Morehouse School of Medicine Atlanta GA 30310 USA;

    MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell Science Hefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of China Hefei 230027 China;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 遗传学分支学科;
  • 关键词

    organoids.cell division; central spindle; CENP-E; syntelin; PRC1;

    机译：有机件.Cell师;中央主轴;CENP-E;SYNTELIN;PRC1;
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