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茶树根际促生菌的筛选与促生特性的研究

         

摘要

从贵州地区的茶树根际土壤中分离筛选出6株根际促生菌,经16S rRNA基因分子鉴定,它们分属于假单胞菌属(Pseudomonas sp.)、芽孢杆菌属(Bacillus sp.)、束村氏菌属(Tsukamurella sp.)、伯克霍尔德氏菌属(Burk ̄holderia sp.)和根瘤菌属(Rhizobium sp.)中。促生特性的研究结果显示,溶磷解钾性能最优的菌株为GD-3菌株,固氮效能最高的KKS-6-N1菌株,此外6个菌株具有溶磷解钾固氮、产IAA、产NH3、产HCN能力、可分泌嗜铁素及具有ACC脱氨酶活性等多种促生性能,可作为研制微生物肥料的优良菌株资源。%Based on sequence conservation of 16S rRNA, six strains of plant growth-promoting rhizobacteria ( PGPR) , which isolated from rhizosphere soils of Guizhou tea trees, were identified as Pseudomonas sp., Bacillus sp., Tsukamurel ̄la sp., Burkholderia sp. and Rhizobium sp. GD-3 showed the most efficiency in phosphatesolubilization and potassium de﹣composition, and KKS-6-N1 had the most efficiency in nitrogen fixing. In addition, other growth-promoting characteris﹣tics, e.g. nitrogen fixation,IAA, NH3, HCNproduction,siderophore secretion and 1-aminocyclopropane-1-carboxylic acid ( ACC) deaminase activity, were also investigated in these six strains, justifying their potentials for development of﹣biological fertilizer as microbial resources in the future.

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