以无菌苗率和优良芽率为主要指标,通过对比不同基本培养基和6 - BA浓度的试验,以探索爬竹侧芽适宜的消毒方法和诱导培养基.结果表明,爬竹侧芽适宜的消毒方法为:75％酒精处理1 min后再用0.1％升汞处理8 ～ 10 min,无菌苗率可达75％左右；采用3/5MS基本培养基时优良芽率最高,显著优于MS、3/4 MS及1/4 MS；在不同激素组合下,6 - BA浓度为2mg/L时优良芽率最高,当6- BA浓度高于2mg／L时,优良芽率呈显著性减小.%The method for explant disinfection and the induction medium for lateral bud of Drepanostachyumrnscandens were optimized in the present work. The results showed that the optimal explant disinfection wasrn75% alcohol for 1 min, followed by 0. 1% HgCl2 for 6-8min, by which around 75% of fungi-free explantsrnmight be gained. Among the tested basic medium, 3/5 MS gave the best effect for vigorous bud induction. 6-BA contributed highly to bud induction, and 2mg/L was the best concentration for strong bud induction.
展开▼
