目的：探讨二氢睾酮（DHT）与骨髓间充质干细胞（BMSCs）联合应用对小鼠外伤性脑损伤（TBI）后海马区脑源性神经营养因子（BDNF）及胶质细胞源性神经营养因子（GDNF）的表达、轴突再生和功能恢复的影响。方法30只成年C57BL／6小鼠随机分为对照组（TBI组）、BMSCs组和DHT ＋BMSCs组。术后28d，NSS法和电生理方法检测运动功能的恢复，West-ern bolt检测海马CA1区BDNF、GDNF及生长相关蛋白43（GAP－43）蛋白表达。免疫荧光检测海马区PKH26标记的移植BMSCs表达。结果与TBI组比较，BMSCs组和DHT ＋BMSCs组海马区BDNF、GDNF及GAP43蛋白相对表达明显增高，电生理波幅增高、神经传导速度增快（ P ＜0．05），其中DHT ＋BMSCs组神经恢复作用显著优于BMSCs组，而且DHT ＋BMSCs组PKH26标记BMSCs细胞数显著高于BMSCs组，NSS神经功能损害评分低于TBI组和BMSCs组（ P ＜0．05）。结论二氢睾酮联合BMSCs移植对小鼠TBI损伤后轴突再生和功能恢复的作用优于BMSCs组，其机制可能与二氢睾酮促进海马区BD-NF及GDNF等神经营养因子表达，进而促进移植的BMSCs存活有关。%Objective To study the effect of dihydrotestosterone ( DHT) and Bone marrow mesenchymal stem cells ( BMSCs) transplantation on expression of BDNF, GDNF and function recovery after traumatic brain injury (TBI) in mice.Methods A total of 30 healthy C57BL/6 mice were randomly divided into Control group, BMSCs group and DHT +BMSCs group.The NSS and electro-physiology were used to evaluate functional recovery.BDNF, GDNF and GAP43 protein in the hippocampus were evaluated by Western bolt, and the fluorescence signal of BMSCs labeled with PKH-26 in the hippocampus was observed by immunofluorescence.Results Compared with control ( TBI) group, the expression of BDNF, GDNF, GAP43 in the hippocampus were increased.Nerve conduc-tion velocity and wave amplitude were significantly increased in BMSCs group and DHT +BMSCs group ( P <0.05).The effect of DHT +BMSCs group was more powerful than BMSCs group ( P <0.05) , moreover, the PKH26 labeled BMSCs of DHT +BMSCs group was higher than that in BMSCs group, the NSS score of DHT +BMSCs group was higher than that in ANA group and BMSCs group ( P <0.05).Conclusion The combined DHT and BMSCs transplantation treatment promoted axon regeneration and function recovery significantly more than BMSCs treatments, which may be related to DHT upregulating BDNF and GDNF expression in the hip-pocampus, and promoting BMSCs survival.
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