目的 探讨第10号染色体同源丢失性磷酸酶-张力蛋白基因(phosphate and tension homology deleted on chromsome ten,PTEN)启动子甲基化与膀胱尿路上皮癌(bladder urothelial cell carcinoma,BUCC)临床特征的关系.方法 选取41例BUCC组织及18例正常膀胱组织标本,采用甲基化特异性PCR(methylation specific PCR,MSP)方法检测PTEN基因启动子甲基化水平,采用Western Blot方法检测PTEN蛋白的表达水平,并分析PTEN基因启动子甲基化水平与BUCC不同临床特征的关系.结果 BUCC中PTEN蛋白的表达低于正常膀胱组织(P=0.031);BUCC中PTEN基因启动子甲基化率为53.66％(22/41),高于正常膀胱组织的0.00％ (0/18) (P=0.000);PTEN基因启动子甲基化在不同性别、年龄患者之间的差异无统计学意义(P=0.149、P=0.813);在不同的临床分期、病理分级及是否有淋巴结转移的患者之间的差异有统计学意义(P=0.008、P=0.021、P=0.038).结论 PTEN基因启动子甲基化水平与BUCC病理分级、临床分期和淋巴结转移有关,而与性别、年龄无关.%Objective To investigate the relationship between PTEN gene promoter methylation and clinical characteristics of bladder urothelial cell carcinoma (BUCC).Methods PTEN gene promoter methylation was detected by methylation-specific PCR and PTEN protein expression was measured by Western Blot in 41 BUCC specimens and 18 normal bladder tissue specimens.The correlation between PTEN gene promoter methylation and clinical characteristics of BUCC was analyzed.Results PTEN protein expression in BUCC was lower than that in normal bladder tissue(P=0.031).PTEN gene promoter methylation rate in BUCC(53.66％,22/41) was higher than that in normal bladder tissues(0％,0/18)(P-0.000).PTEN gene promoter methylation was correlated with clinical stage,pathological grade and lymph node metastasis(P=0.008,P=0.021 and P=0.038,respectively),but was not correlated with the gender and age(P=0.149 and P=0.813,respectively).Conclusion PTEN gene promoter methylation is related to pathological grade,clinical stage and lymph node metastasis of BUCC,but not to gender and age.
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