Objective To explore the effects of ghrelin on the proliferation and apoptosis of hu-man ovarian cancer HO-8910 cells.Methods HO-8910 cells were treated with different concen-trations of ghrelin(400,500,600,700 and 800 ng·mL-1 ).Cell proliferation,apoptosis and phos-phorylated ERK1/2(p-ERK1/2)expression were measured by MTT,TUNEL and Western blot-ting assays,respectively.Results The treatment with ghrelin(600,700 and 800 ng·mL-1 )sig-nificantly inhibited the proliferation HO-8910 cells(P <0.05).Furthermore,the treatment with ghrelin(600 ng·mL-1 )for 20 minutes significantly decreased apoptosis and p-ERK1/2 grayscale value(P <0.05).Conclusion Ghrelin treatment can inhibit proliferation and promote apoptosis in human ovarian cancer HO-8910 cells.In addition,ERK1/2 may be involved in the mechanism of action of ghrelin.%目的：探讨 ghrelin 对人卵巢癌细胞株 HO-8910增殖及凋亡作用的影响。方法将不同质量浓度的 ghre-lin（400、500、600、700、800 ng·mL－1）与人卵巢癌细胞株 HO-8910共同培养，采用 MTT 法检测细胞增殖的抑制率，Tunel 法检测细胞凋亡，蛋白免疫印迹法（Western blotting）检测磷酸化的 ERK1／2（p-ERK1／2）的表达。结果ghrelin≥600 ng·mL－1时对 HO-8910细胞增殖的抑制率具有显著作用（P ＜0．05）。600 ng·mL－1质量浓度的 gh-relin 作用于 HO-8910细胞20 min 后，细胞凋亡、p-ERK1／2的灰度值均显著减少（均 P ＜0．05）。结论ghrelin 能抑制人卵巢癌细胞株 HO-8910增殖、促进其凋亡；ERK1／2可能参与了 ghrelin 对人卵巢癌细胞株 HO-8910作用的过程。
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