OBJECTIVE To establish an ovarian cancer xenograft model by fluorescence and study of the cancer staging. METHODS The human ovarian cancer HO-8910 cells were cultured for 10 generations in the cell culture dish,and the cells that could stably express fluorescence were selected and injected into nude mice.When the tumor grew to a mass volume of 1 mm3 ,it was implanted into the ovarian mucous membrane of nude mice through micro-surgery.The tumor growth and metas-tasis were observed by in vivo imaging technique,and the tumor tissue and surrounding tissues were observed through patholo-gy detection methods.RESULTS In vivo imaging technology successfully traced the development and metastasis of tumor. Pathological examination showed tumor cell metastasis trend.Both of them combining with TCM clinical ovarian cancer staging determined the ovarian cancer staging.CONCLUSION The experiment successfully establishes ovarian cancer orthotopic transplantation model and represents the evolution of clinical oncology.The stages will provide some experimental basis for the study of tumor and the evaluation criterion of antitumor drug effect.%目的：建立 HO-8910卵巢癌荧光原位移植瘤模型,并进行分期研究。方法将人卵巢癌 HO-8910细胞株在细胞培养瓶中培养至10代,选荧光稳定表达的细胞注射到裸鼠皮下,待成瘤后通过显微外科手术将体积为1 mm3肿瘤块植入到实验裸鼠卵巢黏膜上,在活体成像技术下观察肿瘤的生长和转移情况,并应用病理学检测方法对肿瘤组织及附近组织进行观察。结果活体成像技术成功追踪了肿瘤的发展与转移,病理结果显示肿瘤细胞的转移趋势,2者与中医临床卵巢癌分期相结合,确立了卵巢癌分期。结论成功建立卵巢癌原位移植瘤模型,再现了肿瘤临床演变过程。其3期分期为肿瘤研究及抗肿瘤药物疗效判定研究提供了实验基础。
展开▼
