ABSTRACT:OBJECTIVE To explore the effect of Baicalin in vivo of the expression of NF-κB and cytokines in ulcerative coli-tis rats.METHODS The colitis model of mice were established with TNBS methord.Mice were randomly divided into control group,model group,Baicalin group,and SASP group.After continuous administration for 10 days,observed the colon gross morphological changes,the colon tissue level of TNF-α,IL-8 and IL-1 were detected using ELISA,and the protein expression of NF-κB in the colon tissues of mice were detected using Western Blot.RESULTS Compared with the model group,Baicalin(100 mg∕kg) group could significantly improve the colon gross morphological form,the Baicalin(25,50,100 mg∕kg)groups could in-hibit the secretion of inflammatory factors dose-dependently,the high dose group could reduce IL-1,IL-8 and TNF-αexpression (P <0.05),no significant difference occured between the SASP group.Compared with the model group,.Baicalin high dose group could reduce NF-kB levels,promote IkBαlevel(P <0.05) .CONCLUSION Baicalin can inhibit NF-κB activation,and thus play its anti-inflammatory effect in ulcerative colitis mice.%目的：观察黄芩苷对溃疡性结肠炎模型大鼠核因子-κB(Nuclear factor-κB,NF-κB)表达及下游炎症细胞因子的影响。方法采用 TNBS 法制备大鼠溃疡性结肠炎模型,随机分为正常组、模型组、黄芩苷(Baicalin)高中低剂量组、阳性对照组(柳氮磺胺吡啶,SASP 组)。给药10 d 后,留取结肠组织标本,观察结肠大体形态变化,采用 ELISA 法观察 UC 模型大鼠结肠组织中肿瘤坏死因子(TNF-α)、白介素-8(IL-8)和白介素-1(IL-1)的含量,采用 Western blot 法检测 NF-κB 的表达水平。结果与模型组相比,黄芩苷100 mg/kg 组能显著改善结肠大体形态,黄芩苷(25、50、100 mg/kg)干预后能剂量依赖性的抑制各炎症因子的分泌,黄芩苷高剂量组能降低 IL-1、IL-8和 TNF-α表达水平,但与 SASP 组比较无明显差异。黄芩苷高剂量组能降低 NF-κB 的水平,促进 IκBα水平(P <0.05)。结论黄芩苷能够抑制 NF-κB 的活化,从而发挥其在溃疡性结肠炎中的抗炎效应。
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