ABSTRACT:OBJECTIVE To study the excretion of Liguzinediol and its metabolites in rats by UPLC-PDA method.METH-ODS Six SD rats were administered intravenously at a dose of 10 mg∕kg.Its urine,bile and feces were collected according to certain time points.The samples were treated by methanol and the supernatant was dried by N2 ,then dissolved by mobile phase.Liguzinediol and its metabolites in urine,bile and feces were determinated by UPLC-PDA.Conversion factor was con-firmed through the determination of UV absorption coefficient and molecular weight of prototype drug together with its metab-olites.Then the cumulative excretion of Liguzinediol in rats(Dose%)was calculated.RESULTS There were slight differences in the excretion process between female and male rats.The Dose% of Liguzinediol and its main metabolites in female rats u-rine,bile and feces respectively were 47.94%,1 6.67% and 0.648%,and the total Dose% was 65.26%.The Dose% of Ligu-zinediol and its main metabolites in male rats urine,bile and feces respectively were 35.00%,20.37% and 1.1 56%,and the total Dose% was 56.53%.CONCLUSION The UPLC-PDA method with the addition of conversion factor can be used to ex-plore the material balance of Liguzinediol in rats,and provide experimental evidence for clinical research.%目的：采用 UPLC-PDA 法对 Liguzinediol 及其代谢产物在大鼠体内的排泄进行研究。方法 SD 大鼠雌雄各6只,尾静脉注射给药10 mg/kg,按照时间点分别收集尿液、胆汁、粪便,样品经甲醇处理后,取上清液 N2吹干,流动相复溶。采用 UP-LC-PDA 法对大鼠尿液、胆汁、粪便中 Liguzinediol 及其代谢产物进行测定,通过代谢产物与原型药物的吸收系数之比及分子量折算确定代谢产物的换算因子,计算 Liguzinediol 在大鼠体内的累计排泄量占剂量的百分比(Dose%)。结果雌雄大鼠尿液、胆汁及粪便排泄过程略有差异,Liguzinediol 及其主要代谢产物在雌性大鼠的尿液、胆汁及粪便的 Dose%分别为47.94%、16.67%、0.648%,体内累计 Dose%为65.26%；Liguzinediol 及其主要代谢产物在雄性大鼠的尿液、胆汁及粪便的 Dose%分别为35.00%、20.37%、1.156%,体内累计 Dose%为56.53%。结论采用加换算因子的 UPLC-PDA 法可以简便、快速地对Liguzinediol 在大鼠体内的物质平衡进行探索性研究,为开展药物临床研究提供实验依据。
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