ABSTRACT:OBJECTIVE To observe the effects of iridoid glycoside extract from Corni Fructus(ECO)on glucose consump-tion and isoproterenol (ISO)-induced lipolysis in 3T3-L1 mature adipocytes and possible mechanism.METHODS Glucose consumption of mature adipocytes intervened by ECO for 24 h was detected in GOP-POD method.After 2 d of ECO interven-tion,colorimetric method was used to evaluate the release amount of glycerol from 30 min ISO-induced lipolysis.The expres-sion of AMPK,GLUT4 and HSL mRNA were detected by Real-time PCR and phosphorylation levels of AMPK and ACC were evaluated by immunoblot.RESULTS In mature 3T3-L1 adipocytes,1 g/L ECO intervention increased glucose consumption and reduced the glycerol release amout from lipolysis induced by ISO with different concentrations.0.2 g/L and 0.04 g/L ECO decreased 100 nmol/L ISO-induced lipolysis.After 2 d intervention,ECO upregulated the levels of AMPK and GLUT4 mRNA and decreased the gene expression of HSL.1 g/L ECO promoted the phosphorylation of AMPK and ACC.CONCLUSION ECO can increase glucose consumption and inhibited ISO-induced lipolysis in 3T3-L1 mature adipocytes and the effects might be related to AMPK regulation.%目的：观察山茱萸环烯醚萜苷类提取物对3T3-L1成熟脂肪细胞葡萄糖消耗及异丙肾上腺素（ISO）诱导脂肪分解的影响，探讨其分子机制。方法葡萄糖氧化酶法测定0．04、0．2、1 g／L 浓度山茱萸提取物干预24 h 后对脂肪细胞葡萄糖消耗量的影响；比色法测定干预2 d 后 ISO 30 min 诱导脂肪细胞脂肪分解的甘油释放量；Real-time PCR 法检测干预2 d 后脂肪细胞AMPK、GLUT4和 HSL mRNA 表达水平，Western blot 检测药物对 AMPK、ACC 蛋白磷酸化的影响。结果1 g／L 山茱萸提取物干预成熟脂肪细胞24 h 后可增加葡萄糖消耗，干预2 d 后可明显抑制不同浓度 ISO 诱导的脂肪分解，0．2、0．04 g／L 提取物可抑制低浓度 ISO 诱导的脂肪分解；各浓度提取物干预2 d 可不同程度上调 AMPK、GLUT4 mRNA 水平，下调 HSL mRNA 表达，1 g／L 浓度时 AMPK、ACC 磷酸化水平升高。结论山茱萸提取物可能通过上调 AMPK 基因表达的相关途径增加3T3-L1脂肪细胞葡萄糖消耗并抑制 ISO 诱导的脂肪分解。
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