Objective To investigate the effects of propofol on Ca2+ - ATPase activity of myocardial plasma membrane in cardiac hypertrophy rats. Methods Fifty SD rats were randomly divided into normal control group(n = 10) and model of myocardial hypertrophy group(n =40).The myocardial hypertrophy group were randomly divided into four subgroups with 10 rats earch; model group, 30mg propofol group, 60mg propofol group and intralipid group. Forty myocardial hypertrophy model rats were treated with norepinephrine (NE) 1.5mg·kg-1 intraperitoneally twice daily for 15 day. On the 16th day, normal saline, different doses of propofol or intralipid were infused intravenous after anesthesia. Calcium ion concentration and Ca2+ - ATPase activity of myocardial plasma membrane were measured after 30 min infusion. Results Compared with normal control group, calcium ion concentration of each subgroups were significantly higher(P ＜0. 05 ) , however no statistical significance among subgroups of group B(P ＞0.05). Compared with normal control group, Ca2+ -ATPase activity of each subgroups were significantly decreased ( P ＜ 0. 05 ). Among myocardial hypertrophy group : model group and intralipid group were significantly higher than 30mg propofol group and 60mg propofol group which 30mg propofol group was significantly higher than 60mg propofol group (P ＜ 0.05). Conclusion Cardiac hypertrophy of rat myocardial plasma membrane Ca2+ - ATPase activity is related to the dose of propofol , and the activity was reduced as the dose increased.%目的 观察丙泊酚对心肌肥厚大鼠心肌肌浆网Ca2+-ATPase活性的影响.方法 50只雄性SD大鼠采用SAS统计软件随机分为正常对照组(n=10)和心肌肥厚模型组(n=40).心肌肥厚组再随机均分四个亚组(n=10):模型组、丙泊酚30mg组、丙泊酚60mg组和脂肪乳剂组.采用腹腔注射去甲肾上腺素(NE)方法制备大鼠心肌肥厚模型,NE 1.5mg·kg-1腹腔注射,每天2次,连续15d得到心肌肥厚模型.次日麻醉后分别静脉输注生理盐水、不同剂量丙泊酚及脂肪乳,输注30mins后处死大鼠,测量心肌组织Ca2浓度及肌浆网Ca2+-ATPase活性.结果 与正常对照组比较肥厚模型组各亚组心肌组织Ca2+浓度增高(P＜0.05),而肥厚模型组各亚组比较Ca2浓度差异无统计学意义(P＞0.05).与正常对照组比较肥厚模型组各亚组心肌肌浆网Ca2+-ATPase活性降低(P＜0.05),肥厚模型组各亚组比较:模型组、脂肪乳剂组高于丙泊酚30mg组、丙泊酚60mg组,丙泊酚30mg组高于丙泊酚60mg组(P＜0.05),余差异无统计学意义(P＞0.05).结论 心肌肥厚大鼠心肌肌浆网Ca2+-ATPase活性与丙泊酚的剂量有关,随着剂量增大活性降低.
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