Objective To determine whether oxymatrine and matrine can against antigen - challenged vaso-constriction of isolated thoracic aorta in guinea pig, if so, to investigate whether the protective mechanism are related to the activation of the transient receptor potential vanilloid type 1 ( TRPV1) . Methods The thoracic aorta rings of guinea pigs were used to study the inhibitory effect of oxymatrine and matrine on vasoconstriction induced by bovine serum albumin. Results The intense vasoconstrictor response induced by bovine serum albumin was significantly attenuated by diphenhydramine, the H1 receptor antagonist. Oxymatrine (3.3 × 10 mol · L ~3.3×l0- mol · L-1 ) significantly decreased the antigen -challenged vasoconstriction on isolated aortic rings of guinea pigs, and this action was abolished by capsazepine (10 -5 mol · L-1 ) ,a selective TRPV1 antagonist. Matrine (3.3 × 10 mol · L , 1 × 10 mol · L ) could also significantly suppress the vasoconstriction, but its effect was not modified by capsazepine. Conclusion The protective effect of oxymatrine on vasoconstriction is induced by anaphylaxis via activation of TRPV1, whereas the vasodilative effect of matrine is not.%目的 研究苦参碱和氧化苦参碱对抗原攻击所致血管收缩的影响.方法 腹腔注射牛血清白蛋白建立预致敏的豚鼠模型,21d后制备离体豚鼠主动脉环,用0.04mg·mL-1牛血清白蛋白诱发血管过敏反应,记录血管张力的改变.观察苯海拉明、苦参碱和氧化苦参碱对抗原攻击所致血管收缩的影响,并观察capsazepine等对不同剂量苦参碱和氧化苦参碱作用的影响,分析其对血管过敏性损伤保护作用的机制.结果 抗原攻击能诱导豚鼠预致敏的离体胸主动脉环收缩,苯海拉明(H1受体阻断剂)能显著抑制抗原攻击所致的血管收缩;氧化苦参碱(3.3×10-4～3.3×10-3 mol·L-1)能显著降低抗原攻击所致血管的收缩效应,瞬时受体电位香草酸亚型(Transient Receptor Potentiol Vanilloid 1,TRPV1) 受体阻断剂capsazepine(10μmol·L-1)能消除氧化苦参碱对血管收缩的抑制效应;苦参碱(1×10-3、3.3×10-3mol·L-1)能显著降低抗原攻击所致血管的收缩效应,此作用不受capsazepine(10μmol·L-1)的影响.结论 氧化苦参碱能显著抑制血管过敏反应时血管的收缩,其作用与激活TRPV1 受体有关;较高浓度苦参碱也能显著抑制血管过敏反应时血管的收缩,其作用与TRPV1 受体无关.
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