首页> 中文期刊> 《东北农业大学学报:英文版》 >Cloning and Sequence Analysis of Y-box Binding Protein Gene in Min Pig

Cloning and Sequence Analysis of Y-box Binding Protein Gene in Min Pig

         

摘要

In order to study the gene sequence of Min pig Y-box binding protein(YB-1)gene,the complete coding sequence of Min pig YB-1 gene was cloned by RT-PCR,the sequence features were analyzed by some software and online website.The results showed that the complete CDS of Min pig Y-box was found to be 975 bp long,encoding 324 amino acids.It contained a conserved cold shock domain and several phosphorylation sites,but had no transmembrane domains,and was consistent with a protein found in the cytoplasm.Min pig YB-1 nucleotides shared high similarity(61.37%-97.66%)with other mammals.

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