To investigate the effects of Codonopsis pilosula(CPPS) on the liver and spleen histolomorph structure and the expression of Bax and VEGF in liver and spleen of mouse induced by D‐galactose , 100 mouse(Kunming) are divided into normal control group ,aging model group and low/middle/high dose of CPPS inerventional groups randomly . After treatment for 42 days , the changes of the liver coefficient and spleen coefficient are recorded , the morphologic changes of these tissues are observed , and the changes in the expression of Bax and VEGF in liver and spleen are also determined by immunohistochemical method . Results show that the liver coefficient and spleen coefficient of aging model group are significantly decreased(P< 0.05) , and markedly swelled , surface is rough and presenting bright red . The positive expression of Bax protein is increased significantly in liver and spleen of aging model group , w hile positive expression of VEGF protein is decreased significantly (P<0.05) .Compared with aging model group ,the organ coefficients in the high dose of CPPS interventional group are significantly increased (P<0.05) , the liver swollen is significantly mitigated ,and surface is smooth . The positive expression of Bax protein is decreased significantly in liver and spleen of high dose of CPPS interventional group , w hile the positive expression of VEGF protein is increased significantly ( P< 0.05 ) . All results indicate that CPPS can stabilize the histolomorph structure , inhibit the excessive apoptosis of cells , and promote angiogenesis , w hich can protect the liver and spleen of aging mouse induced effectively .%为研究党参水提物对D‐半乳糖致衰老小鼠肝脏和脾脏组织结构及Bax蛋白及V EG F表达的影响，将100只昆明小鼠随机分为对照组、D‐半乳糖致衰老模型组、低、中、高剂量党参干预组．连续处理42 d ，随时监测小鼠的体重变化，处理结束后，称取脏器质量，观察组织显微结构，并用免疫组织化学法检测Bax蛋白和V EG F在肝脏和脾脏组织的表达．结果显示衰老组小鼠肝脏和脾脏指数明显下降（P＜0.05）．多数肝细胞水肿，胞浆空淡，呈气球样变．脾窦明显扩张，红髓范围扩大，脾小梁明显增生．Bax蛋白表达明显增强， VEGF蛋白表达显著减少．与衰老组相比，党参干预高剂量组的脏器指数明显增加（P＜0.05），肝小叶结构清楚，肝细胞体积增大，肝细胞水肿明显减轻．白髓红髓清楚，红髓范围减小，脾小梁增生不明显．Bax蛋白表达明显减少， VEGF蛋白表达明显增加．表明党参水提物可以稳定肝脾的组织结构，抑制细胞凋亡，促进血管生成，对衰老小鼠的肝脏和脾脏组织有一定的保护作用．
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