以千日红种子为外植体，获得无菌苗，并采用正交试验设计方法进行无菌苗的增殖、生根壮苗培养和试管开花诱导．无菌苗最佳增殖培养基为： MS ＋1.0 mg・L -1 BA ＋0.5 mg・L -1 NAA ，培养28 d ，增殖系数为3.1，苗高可达3.3 cm ；最佳生根培养基为：1/2 MS ＋0.5 mg・L -1 IBA ＋30 g・L -1蔗糖＋0.5 g・L -1活性炭，并以透气膜为封口材料；无菌苗接种于改良 MS ＋5.0 mg・L -1 PP333＋40 g・L -1蔗糖＋7 g・L -1亚精胺，平均开花率达11.11％．采用组织培养方法获得无菌苗，并诱导其在试管内开花，可为其优良品种的快速繁殖和开发利用提供技术支持．%Seeds of Gomphrena globosa are acted as experimental materials to get aseptic seedling and then used for multiplication , rooting and flowering in vitro by orthogonal design . Results show that the optimal multiplication medium is : MS + 1.0 mg・L - 1 BA + 0.5 mg・L - 1 NAA , after 28 days of culture , the multiplication coefficient is 3.1 and the average seedling height reach to 3.3 cm . The optimized medium for rooting is 1/2 MS + 0.5 mg・L - 1 IBA + 30 g・L- 1 sucrose + 0.5 g・L- 1 activate charcoal with breathable film as sealing materials . The medium for flowering is modified MS + 5 mg・L - 1 PP333 + 40 g・L- 1 sucrose + 7 mg ・ L- 1 spermidine ,with the average flowering rate is 11.11% . Aseptic seedling is obtained by tissue culture and then induced in vitro flowering , which is useful to provide technical support for the development and utilization of Gomphrena globosa by rapid propagation .
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