土壤盐碱化是引起土壤生态环境恶化的主要原因之一,盐碱土的改良利用是目前研究的热点.本文采用构建16S rRNA基因文库,研究了位于中国西北部的河西走廊盐碱土中细菌群落结构多样性.结果表明,原生盐碱土、次生盐碱土和农田土三种不同类型的土壤中细菌种群结构的组成和丰度具有明显差异.作为对照的农田土中存在3大细菌类群,其中Firmicutes为优势菌群；原生盐碱土中存在9大细菌类群,Firmicutes和γ-proteobacteria为优势菌群；三个次生盐碱土样品存在很大差异,γ-proteobacteria为其共同优势菌群.Marinobacter,Halomonas和Pseudomonas这些嗜盐菌普遍存在于盐碱土中.%Salinization of soil is one of the main reasons causing the deterioration of soil ecological environment, and the remediation and utilization of saline-alkali soil is the focus of current research. By building 16S rRNA gene library, this article reveal the diversity of bacterial population structure at the saline-alkali soil in the Hexi corridor in northwest China. Statistical analyses show that the significant difference in the composition and abundance of bacterial population structure exist in the primary saline-alkali soil, the secondary saline alkali soil and farmland soil. Three bacterial groups exist in the control soil, Firmicutes is the dominant population; Nine bacterial groups exist in the primary saline-alkali soil, Firmicutes and y-proteobacteria are the dominant population; existing a significant difference between the three samples of the secondary saline-alkali soil, y-proteobacteria is the common dominant bacteria group. The halophilic bacteria such as Marinobacter, Halomonas and Pseudomonas commonly are found in all saline-alkali soil.
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