首页> 中文期刊> 《海洋湖沼学报(英文)》 >Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer(ITS) region of peritrich ciliates in environmental samples

Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer(ITS) region of peritrich ciliates in environmental samples

         

摘要

cqvip:Peritrich ciliates are highly diverse and can be important bacterial grazers in aquatic ecosystems. Morphological identifi cations of peritrich species and assemblages in the environment are time-consuming and expertise-demanding. In this study, two peritrich-specifi c PCR primers were newly designed to amplify a fragment including the internal transcribed spacer(ITS) region of ribosomal rDNA from environmental samples. The primers showed high specifi city in silico, and in tests with peritrich isolates and environmental DNA. Application of these primers in clone library construction and sequencing yielded exclusively sequences of peritrichs for water and sediment samples. We also found the ITS1, ITS2, ITS, D1 region of 28 S rDNA, and ITS+D1 region co-varied with, and generally more variable than, the V9 region of 18 S rDNA in peritrichs. The newly designed specifi c primers thus provide additional tools to study the molecular diversity, community composition, and phylogeography of these ecologically important protists in dif ferent systems.

著录项

  • 来源
    《海洋湖沼学报(英文)》 |2018年第3期|P.818-826|共9页
  • 作者

    苏蕾; 张倩倩; 龚骏;

  • 作者单位

    [1]Laboratory of Microbial Ecology and Matter Cycles;

    Yantai Institute of Coastal Zone Research;

    Chinese Academy of Sciences;

    Yantai 264003;

    China;

    [2]University of Chinese Academy of Sciences;

    Beijing 100049;

    China;

    [1]Laboratory of Microbial Ecology and Matter Cycles;

    Yantai Institute of Coastal Zone Research;

    Chinese Academy of Sciences;

    Yantai 264003;

    China;

    [2]University of Chinese Academy of Sciences;

    Beijing 100049;

    China;

    [1]Laboratory of Microbial Ecology and Matter Cycles;

    Yantai Institute of Coastal Zone Research;

    Chinese Academy of Sciences;

    Yantai 264003;

    China;

    [2]University of Chinese Academy of Sciences;

    Beijing 100049;

    China;

  • 原文格式 PDF
  • 正文语种 CHI
  • 中图分类 湖泊、水库;
  • 关键词

    富钙纤毛虫; 水生生态系统; 理论研究; 湖泊;

    机译:富钙纤毛虫;水生生态系统;理论研究;湖泊;
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