Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system

HOU Dandan; YU Weiting; ZHANG Demeng; ZHAO Lili; LIU Xiudong; MA Xiaojun

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Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system

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摘要

Immobilization biocatalysis is a potential technology to improve the activity and stability of biocatalysts in nonaqueous systems for efficient industrial production.Alginate-chitosan(AC)microcapsules were prepared as immobilization carriers by emulsifi cation-internal gelation and complexation reaction,and their contribution on facilitating the growth and metabolism of yeast cells were testifi ed successfully in culture medium-solvent biphasic systems.The cell growth in AC microcapsules is superior to that in alginate beads,and the cells in both immobilization carriers maintain much higher activity than free cells,which demonstrates AC microcapsules can confer yeast cells the ability to resist the adverse effect of solvent.Moreover,the performance of AC microcapsules in biphasic systems could be improved by adjusting the formation of outer polyelectrolyte complex(PEC)membrane to promote the cell growth and metabolic ability under the balance of resisting solvent toxicity and permitting substrate diffusion.Therefore,these findings are quite valuable for applying AC microcapsules as novel immobilization carriers to realize the biotransformation of value-added products in aqueous-solvent biphasic systems.

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来源
《海洋湖沼学报（英文）》 |2019年第3期|P.863-870|共8页
作者
HOU Dandan; YU Weiting; ZHANG Demeng; ZHAO Lili; LIU Xiudong; MA Xiaojun;
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作者单位

[1]College of Environment and Chemical Engineering;

Dalian University;

Dalian 116622;

China;

[2]Affiliated Zhongshan Hospital of Dalian University;

Dalian 116001;

China;

[3]State Key Laboratory of Bioactive Seaweed Substances;

Qingdao Brightmoon Seaweed Group Co.Ltd.;

Qingdao 266400;

China;

[3]State Key Laboratory of Bioactive Seaweed Substances;

Qingdao Brightmoon Seaweed Group Co.Ltd.;

Qingdao 266400;

China;

[1]College of Environment and Chemical Engineering;

Dalian University;

Dalian 116622;

China;

[4]Dalian Institute of Chemical Physics;

Chinese Academy of Sciences;

Dalian 116023;

China;

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原文格式 PDF
正文语种 CHI
中图分类天文学、地球科学;
关键词
alginate-chitosan(AC)microcapsules; immobilization biocatalysis; aqueous-solvent biphasic system; cell growth;

机译：海藻酸盐-壳聚糖（AC）微胶囊;固定化生物催化;水-溶剂双相系统;细胞生长;

Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system

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