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首页> 中文期刊> 《植物资源与环境学报》 >基于psbA-trnH分析的何首乌野生居群遗传多样性

基于psbA-trnH分析的何首乌野生居群遗传多样性

         
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摘要

Sequence of psbA-trnH of 85 individuals in 17 wild populations of Fallopia multiflora (Thunb.) Harald. from different provinces and regions in China was amplified and analyzed, and on the basis, genetic diversity among populations was analyzed and cluster analysis of 85 individuals was also carried out by NJ method. The results show that the length of psbA-trnH sequence of 85 individuals is 384 bp, in which, there are 167 bp variable sites and 53 bp parsimony informative sites, accounting for 43.5% and 13. 8% of the total length of sequence, respectively. Variable types are mainly base deletion and substitution. Variable sites mainly concentrate in the region of 235-281 bp. 17 populations are almostly divided into three types according to site variation status. The genetic distances among 17 populations are 0.000-0. 172, in which, genetic distances between Guizhou population and other 16 populations are 0.167-0. 172, and those among other 16 populations are 0. 000-0. 017. Nucleotide diversity index (Pi), coefficient of gene differentiation (Nst) and gene flow (Nm) among 17 populations are 0.028 56, 0.918 68 and 0. 04, respectively. Pi, Nst and Nm among other 16 populations except Guizhou population are 0. 015 68, 0. 837 19 and 0. 10, respectively. And Pi, Nst and Nm between Guizhou population and its neighboring populations (Sichuan, Yunnan, Guangxi, Hu' nan and Hubei) are 0.047 99, 0.937 62 and 0.03, respectively. On NJ phylogenetic tree, 17 populations are clustered into four branches and individuals tested in most populations are clustered in a same branch and only Guizhou population is clustered alone in a branch, which is basically same with the deviation result by sequence analysis. It is suggested that 91. 868% of overall genetic variation of 17 wild populations exists among populations and 8. 132% within populations, and gene exchange among populations is less. Except Cuizhou population, overall genetic diversity level among other 16 populations is low, indicating that the overall diversity level of F. multiflora populations is affected by Guizhou population at a large extent.%对产自不同省区的何首乌[ Fallopia multiflora (Thunb.) Harald.] 17个野生居群85个单株的sbA-trnH序列进行了扩增和分析,在此基础上分析了居群间的遗传多样性,并采用NJ法对85个单株进行了聚类分析.结果表明:供试85个单株的psb A-trnH序列长度为384 bp;其中,变异位点为167 bp,简约信息位点为53 bp,分别占序列总长度的43.5％和13.8％.变异类型主要为碱基缺失和替换；变异位点主要集中在235～281 bp区域,根据位点变异情况可将17个居群大体分为3类.各居群间的遗传距离为0.000～0.172,其中,贵州居群与其他16个居群间的遗传距离为0.167 ～0.172,而其他16个居群间的遗传距离为0.000～0.017.17个居群间的核苷酸多样性指数(Pi)、遗传分化系数(Nst)和基因流(Nm)分别为0.028 56、0.918 68和0.04；除贵州居群外其他16个居群的Pi、Nst和Nm分别为0.015 68、0.837 19和0.10；贵州局群与其周边省区(四川、云南、广西、湖南和湖北)居群的Pi、Nst和Nm分别为0.047 99、0.937 62和0.03.在NJ系统树上,17个居群可聚为4支,且大部分居群的供试单株聚在同一分支中；仅贵州居群单独聚为一支,与序列分析的划分结果基本一致.由研究结果可见:野生何首乌居群总遗传变异的91.868％来自居群间,8.132％来自居群内,居群间的基因交流较少；除贵州居群外其余16个居群的整体遗传多样性水平偏低,说明何首乌居群整体多样性水平在很大程度上受贵州居群的影响.

著录项

  • 来源
    《植物资源与环境学报》 |2012年第2期|36-44|共9页
  • 作者

    白明明; 孙小芹; 郭建林; 李密密; 杭悦宇;

  • 作者单位

    江苏省·中国科学院植物研究所(南京中山植物园)江苏省植物迁地保护重点实验室,江苏南京 210014;

    江苏省·中国科学院植物研究所(南京中山植物园)江苏省植物迁地保护重点实验室,江苏南京 210014;

    江苏省·中国科学院植物研究所(南京中山植物园)江苏省植物迁地保护重点实验室,江苏南京 210014;

    江苏省·中国科学院植物研究所(南京中山植物园)江苏省植物迁地保护重点实验室,江苏南京 210014;

    江苏省·中国科学院植物研究所(南京中山植物园)江苏省植物迁地保护重点实验室,江苏南京 210014;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 植物生物化学实验;其他;
  • 关键词

    何首乌; 居群; sbA -trnH序列; 遗传多样性; NJ系统树;

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