Taxol content from the branches and leaves of Taxus chinensis var. Mairei (Lemeé et Lévl.) Cheng et L. K. Fu and its cell cultures was analyzed by using Nova-Pak C18 column and improving the proportion of mobile phase (methanol/ acetonitrile/ water) and modifing column temperature at a wavelength of 227 nm. The improved HPLC method is celerity, nicety and delicacy. The lowest detectable quantity of taxol could reach 0.005 μg.
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