Objective To evaluate the effects of total glucosides of paeony (TGP) on cell proliferation of human HaCaT keratinocytes and the expression of ICAM-1, and explore the signaling pathways which may be involved in the process. Methods HaCaT cells were stimulated by 500 U/mL IFN-γ, then incubated with TGP of various concentrations (0.5 to 312.5 μg/ml). MTT assay was performed to detect the cell proliferation of HaCaT cells. The expression of ICAM-1 in HaCaT cells was evaluated by enzyme linked immunosorbent assay (ELISA) and lfuorescent immunohistochemical technology. Results The proliferation of HaCaT cells was promoted by TGP of low concentrations (0.5 to 25.0μg/ml), but inhibited by TGP of high concentration (62.5 to 125.0μg/ml). TGP of low concentrations (0.5 to 25.0μg/mL) signiifcantly reduced the expression of ICAM-1. Conclusion TGP signiifcantly inhibited the upregulated ICAM-1 secretion of HaCaT cells which induced by IFN-γ, that may be one of the anti-inlfammatory mechanism of TGP.%目的：探讨白芍总苷(TGP)对人永生化角质形成细胞（HaCaT细胞）增生及分泌细胞间黏附分子(ICAM)-1的影响，探讨可能涉及的信号传导通路。方法用500 U/ml干扰素(IFN)-γ诱导HaCaT细胞，不同浓度TGP（0.5～312.5μg/ml）作用于HaCaT细胞。四甲基偶氮唑盐(MTT)法观察TGP对HaCaT细胞增生活性的影响。酶联免疫吸附试验（ELISA）及荧光免疫组化法检测HaCaT细胞表达ICAM-1的水平。结果 TGP在低浓度（0.5～25.0μg/ml）时对HaCaT细胞增生活性有促进作用，在高浓度（62.5～125.0μg/ml）时对HaCaT细胞增生活性呈抑制作用。0.5～25.0μg/ml TGP可显著降低HaCaT细胞表达ICAM-1的水平（P＜0.05，P＜0.01）。结论 TGP对IFN-γ上调HaCaT细胞分泌ICAM-1有显著的抑制作用，可能是TGP抗炎作用机制之一。
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