Objective:To observe the biological characteristics of the adipose-derived stem cells (AD-SCs) differentiated into adipogenic cells, to investigate the feasibility of ADSCs as the seed cells in treatment of pelvic organ prolapse (POP). Methods:ADSCs were isolated and cultured in vitro,and the morphological changes were observed by phase-contrast microscope. The expressions of surface antigenes and the cell cycle were detected by flow cytometry. After induced by adipogenic inducing factor, the differentiations to ad-ipocytes were verified by oil red "O" staining. Results .ADSCs propagated rapidly in vitro, and constituted a homogenous fibroblast-like morphology, the shapes of cells were polygonal,and spindle; 90.60% ADSCs were G0/G1 phase cells; the positive rates of cellular surface antigenes were CD44 99.88%,CD105 95.00% ,CD90 99. 74% ,CD49d 89.56% ,CD34 3. 04% ,CD106 0. 85% ,CD45 0.26%;after induction, intra-cellular lipid droplets could be observed and present jacinth by Oil Red stain in 14 days. Conclusions: ADSCs are easily isolated and cultured in vitro, and have the potential to differentiate into adipogenic cells. ADSCs can be as the seed cells for the treatment of POP.%目的:观察大鼠脂肪源性干细胞(ADSCs)向成脂诱导分化的生物学特性,以探讨ADSCs在治疗盆腔脏器脱垂(POP)中生物工程学的意义.方法:酶消化法体外分离培养大鼠ADSCs；相差显微镜观察ADSCs的细胞形态；运用流式细胞术鉴定细胞周期以及细胞表面抗原的表达；对ADSCs进行成脂诱导分化,油红“O”染色观察其向脂肪细胞分化的能力.结果:体外培养的ADSCs增殖迅速,呈成纤维细胞样生长,细胞呈多角形、长梭形,形态均一；细胞周期结果显示G0/G1期细胞占90.60％；表面抗原阳性表达率分别为CD44 99.88％,CD90 99.74％,CD105 95.00％,CD49d 89.56％,CD34 3.04％,CD106 0.85％,CD45 0.26％；成脂诱导实验细胞内出现脂滴,诱导14天后油红“O”染色呈橘红色.结论:ADSCs易于分离培养,具有向成脂诱导分化的功能,提示ADSCs可作为治疗POP的种子细胞.
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