Objective:To observe the expression of wnt1 in patients with oral submucous fibrosis(OSF) before and after treatment.Methods:40 patients with OSF were treated with triamcinolone acetonide combined with salvia miltiorrhiza,Before and after 4 weeks treatment,pain score of VAS and mouth opening(MO) were examined.wnt1 protein in saliva and gingival crevicular fluid(GCF) was examined by ELISA,wnt1 mRNA expression in buccal mucosa tissue was examined by real-time fluorescent quantitative PCR.20 healthy subjects were served as the controls.Results:The expression of wnt1 in OSF group[buccal tissue RT-PCR (36.89 ± 10.40) × 10-5,saliva ELISA (61.61 ± 4.45) ng/L,GCF ELISA (56.20 ± 3.65) ng/L] were significantly higher than that of control group [buccal tissue RT-PCR (4.63 ± 1.53) × 10-5,saliva ELISA (40.26 ± 3.00) ng/L,GCF ELISA (53.45 ± 1.74) ng/L)] (P ＜ 0.01).In OSF group,after treatment VAS was decreased(P ＜0.01),MO increased(P ＜0.01)),Buccal mucosa wnt1 mRNA level was positively correlated with wnt1 protein in saliva and GCF,negativity with MO (P ＜ 0.05),saliva wnt1 was positively correlated with VAS and GCF wnt1,negitively with MO(P ＜ 0.05).Conclusion:Wnt1 might take part in the occurrence and development of OSF.The detection of wnt1 in saliva and GCF might be a noninvasive method for the evaluation of OSF treatment.%目的:研究wnt1在口腔黏膜下纤维性变(OSF)患者治疗前后的表达并探讨其在OSF发生发展中的作用.方法:40例OSF中期患者采用曲安奈德+丹参局部封闭治疗4周,治疗前及第5周复诊时记录VAS及张口度,取唾液及龈沟液ELISA检测wnt1表达,实时荧光定量PCR检测OSF病变颊黏膜中wnt1 mRNA表达.结果:治疗前OSF组wnt1表达[颊黏膜定量PCR(36.89±10.40)×10-5,唾液浓度(61.61 ±4.45)ng/L,龈沟液浓度(56.20±3.65) ng/L]均高于正常组[颊黏膜定量PCR(4.63±1.53)×10-5,唾液浓度(40.26 ±3.00) ng/L,龈沟液浓度(53.45±1.74) ng/L] (P＜0.01);OSF组经治疗后,唾液与龈沟液中wnt1表达均降低,且与OSF临床严重程度呈正相关(P＜0.05).结论:wnt1可能参与OSF的发生发展,其在唾液与龈沟液中的实时监测有望成为评价OSF诊疗的无创性检控手段之一.
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