目的:探讨芦荟(AVE)提取物对脂多糖(LPS)诱导人牙周膜细胞(hPDLCs)炎性反应的作用.方法:将hPDLCs分为对照组,模型组(1 μg/ml LPS)和AVE组,浓度为0.05,0.1,0.2 mg/ml的AVE分别处理AVE组的hPDLCs;MTT法测定各组细胞存活率;ELISA法检测细胞上清中IL-6含量;用免疫细胞化学染色检测TLR4的表达情况;免疫荧光染色检测NF-κB-p65的核转情况.结果:各组细胞存活率差异没有显著性,模型组上清液中的IL-6含量显著升高,TLR4表达及NF-κB-p65的核转均明显上调;与模型组相比,芦荟提取物呈剂量依赖性抑制细胞上清液中IL-6的分泌,下调TLR4表达及NF-κB-p65的核转移.结论:芦荟提取物能有效抑制人牙周膜细胞的炎症反应,其机制与TLR4/NF-κB-p65信号通路有关.%Objective:To explore the effects of aloe vera extra (AVE) on lipopolysaccharide (LPS)-induced inflammatory response of human periodontal ligament cells(hPDLCs).Methods:hPDLCs were induced with LPS at 1 μg/ml for the simulation of periodontitis model(model group) and then treated by AVE at 0.05,0.1,0.2 mg/ml respectively(AVE group).Cell viability was examined by MTT assay.The level of interleukin-6(IL-6) from cell culture medium was measured by ELISA.The expression of Toll like receptor 4(TLR4) protein was detected by immunocytochemistry staining and the transfer of nuclear factor-kappa B p65 (NF-κB-p65) was observed by immunofluorescence staining.Results:There was no significant difference of the cell viabilities among the groups.IL-6 in culture medium,the expression of TLR4 protein and the transfer of NF-κB-p65 into the nucleus were increased in model group.AVE at 0.05-0.2 mg/ml inhibited the secretion of IL-6 in the cell culture supernatant down-regulated the TLR4 expression,attenuated the transfer of NF-κB-p65 into the nucleus in a concentration-dependent manner.Conclusion:Aloe vera extract can inhibit the inflammation response of hPDLCs induced by LPS through TLR4/ NF-κB-p65 signaling pathway.
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