目的：观察3种排龈线在不同浸提时间下的细胞毒性。方法：用 DMEM细胞培养液制备肾上腺素排龈线、硫酸铝排龈线、不含药排龈线分别浸提5、10、15、30 min 的浸提液，用于培养人牙龈成纤维细胞（HGFs）。采用 MTT 比色法观察 HGFs 增殖活性；Annexin／PI 双染法检测 HGFs 凋亡。结果：3种排龈线的不同浸提时间浸提液都可抑制 HGFs 增殖，诱导 HGFs 凋亡，随着浸提时间延长（15、30 min），HGFs 的早期凋亡率逐渐增加，显著高于空白对照组（P ＜0．05）。结论：3种排龈线具有一定程度时间依赖性细胞毒性。%Objective:To evaluate the toxic effect of 3 different gingival retraction cords.Methods:DMEMextraction of DL-adren-aline HCl,aluminium sulphate and non-drug retraction cords with the extraction time of 5,10,15 and 30 min were respectively pre-pared and were used to culture human gingival fibroblasts(HGFs)in vitro respectively.Cell proliferation was tested by MTT assay. Cell apoptosis was examined by Annexin/PI method.Results:The 3 gingival retraction cord extractions inhibited the roliferation,pro-moted the apoptosis of HGFs(P <0.05),the effects were related to the extraction time.Conclusion:The 3 retraction cords have time-dependant cytotoxity.
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机译：Allelopathic Effects of Aqueous Extracts of Different Tissues and Rhizosphere Root of Adzuki Bean on Growth of Wheat Seedlings红小豆不同组织及根际土壤水浸提液对小麦幼苗生长的影响
