Objective:To study the effect of IL-24 on the differentiation and function of osteoclasts.Methods:Mature osteoclasts were isolated from long bones of neonate rats.Optimal multiplicity of infection(MOI)of AdCMV-EGFP was determined.Then osteo-clasts and RAW264.7 cells were transfected with AdCMV-EGFP and AdCMV-IL-24 respectively.The function of osteoblasts was studied by the observation of bone resorption lacunae,the differentiation of RAW264.7 cells was evaluated by the expression of osteo-clast related genes examined with real-time PCR.Results:Osteoclasts were TRAP positive with more than 2 neclei.MOI=400 was suitable for AdCMV-EGFP transfection.The resorption lacunae area in AdCMV-IL24 transfected cells was larger than that in AdCMV-EGFP transfected cells(P<0.05).Real-time PCR showed that under induced conditions,osteoclastic related genes NFATc1,CTSK and TRAP were changed in RAW264.7 cells transfected with AdCMV-IL-24.Conclusion:IL-24 may promote the differentiation and function of osteoclasts.%目的：研究白细胞介素-24（interleukin-24，IL-24）对破骨细胞分化及功能的影响。方法：从新生24 h内大鼠四肢长骨分离鉴定破骨细胞；确定AdCMV-EGFP的最佳感染复数（MOI），分别转染AdCMV-EGFP和AdCMV-IL-24，培养在骨片上，测定骨吸收陷窝的面积；real-time PCR方法检测在诱导条件下RAW264．7细胞转染AdCMV-IL-24后，破骨细胞相关因子的表达。结果：破骨细胞胞内可见2个以上的细胞核，TRAP染色呈紫红色；MOI＝400为最佳转染效率；转染AdCMV-IL24的破骨细胞骨吸收陷窝面积比转染AdCMV-EGFP的细胞大（P＜0．05）；在诱导条件下RAW264．7细胞转染IL-24后，破骨细胞的相关基因NFATc1、CTSK和TRAP表达发生变化。结论：IL-24能促进破骨细胞的分化，提高破骨细胞的骨吸收功能。
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