Objective:To investigate the clinical significance of epidermal growth factor receptor(EGFR),proliferating cell nuclear antigen(PCNA),laminin(LN)and type IV collagen expression in salivary adenoid cystic carcinoma(SACC).Methods:EGFR gene in 78 cases of SACC with complete clinical data was detected by fluorescence in situ hybridization(FISH)technique,the expression of EGFR,PCNA,LN and type IV collagen protein was detected by immunohistochemistry technique(IHC),their correlation with the clin-icopathological parameters was analysed by SPSS 13.00 software.Results:EGFR gene amplification levels(69.2%)was positively related to the ratio of EGFR protein positive expression(7 1 .8%),the expression of EGFR,PCNA,LN and type IV collagen was posi-tively related to the clinical pathological parameters(P<0.05).There was a positive correlation between EGFR and PCNA expression (P<0.05),a negative correlation between LN protein and type IV collagen protein expression(P<0.05).Conclusion:EGFR gene is amplified in SACC.EGFR,PCNA,LN and type IV collagen take part in the occurrence and development of SACC.%目的：探讨表皮生长因子受体（EGFR）、增殖细胞核抗原（PCNA）、层黏连蛋白（LN）和IV型胶原蛋白在唾液腺腺样囊性癌（SACC）中的表达及临床意义。方法：选取临床病例资料齐全的SACC 78例，用荧光原位杂交技术检测EGFR基因表达，免疫组织化学技术检测 EGFR、PCNA、LN和Ⅳ型胶原蛋白的表达，分析其与临床病理参数的相关性。结果：SACC中EG-FR基因扩增率（69．2％）与蛋白的阳性表达率（71．8％）存在明显正相关（P＜0．05），且EGFR、PCNA、LN、Ⅳ型胶原蛋白表达与临床病理参数密切相关。EGFR、PCNA表达水平间存在明显正相关（P＜0．05）；LN蛋白、Ⅳ型胶原表达水平间存在明显负相关（P＜0．05）。结论：EGFR基因在SACC中明显扩增，EGFR、PCNA、LN、Ⅳ型胶原蛋白共同参与SACC发生、发展。
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