目的：探讨丹参多酚酸盐（DSSM）对高正加速度（＋Gz）重复作用下的大鼠磨牙牙髓的作用。方法：27只 SD 雄性大鼠用随机区组法分为9组，每组3只。阳性对照组和药物组分别在＋5 Gz、＋10 Gz 的条件下共离心30 s，每天重复5次，间隔以＋1Gz 持续60 s，4 d /周，共3周。实验期间药物组于每次离心前30 min 按剂量腹腔注射 DSSM溶液（低、中、高给药剂量分别为2、6、18 mg/kg），阴性及阳性对照组注射等体积生理盐水。实验结束后，光镜下观察各组大鼠牙髓组织形态学变化情况，并采用 Real time qPCR 方法测定大鼠磨牙牙髓中热休克蛋白（HSP70）mRNA 相对含量表达的情况。结果：＋5 Gz 和＋10 Gz阳性对照组大鼠活动程度降低，磨牙牙髓中出现空泡性变，组间差异不明显。HSP70 mRNA 的相对含量比阴性对照组高（P ＜0．05）。药物组中 HSP70 mRNA 的相对表达量较各自阳性对照组降低，＋10 Gz 高剂量组有统计学意义（P ＜0．05）。结论：＋Gz可引起牙髓损伤，＋Gz 值的增加与 HSP70 mRNA 相对表达量增加相关，DSSM可能对牙髓有保护作用。%Objective:To investigate effects of depside salt from salvia miltiorrhiza(DSSM)on the dental pulp of rats under repeated exposure to +Gz acceleration.Methods:27 male SD rats were randomly divided into 9 groups(n =3).The rats in negative control group was only fixed with rats kept-keeling device,in positive control group were exposed to +5 Gz and +10 Gz respectively for 30 s each time,5 times per day,4 days per week,for 3 consecutive weeks.The rats in other groups were respectively given DSSMat 2,6 and 18 mg/kg respectively 30 min before each +Gz exposure.The rats in negative and positive control groups were injected with 0.9%sodium chloride solution 30 min prior to +Gz exposure.After the experiment,The pulp changes were observed by HE staining.Real time qPCR was applied to determine the relative expression of heat sheck protein(HSP70)mRNA.Results:+Gz exposure decreased the activity degree of the rats,and resulted in damage of rat dental pulp.With the increase of +Gz value,the relative expression of HSP70 mRNA was increased(P <0.05).HSP70 mRNA expression in drug groups was less than that in their own positive control groups,but showed statistical significance only in the 18 mg/kg and +10 Gz group(P <0.05).Conclusion:+Gz exposure may dam-age dental pulp.DSSMhas certain protective effect under +Gz exposure.
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