Objective: To investigate the relevance of interleukin-1 (3( IL-1 (3 ) to orthodontic root resorption. Methods: Animal models of root resorption were established in dogs. Semi- quantitative detection by PCR was used to measure the expression of IL-1 (3 mRNA in the periodontal tissues with root resorption and the normal tissues of dogs. The cultured human periodental ligment cells( hPDLCs ) were exposed to IL-1 (3 at 0.01, 0. 1, 1, 10( experimental groups ) and 0 ng/ml( control group ) for 6, 12 and 24 h respectively, PCR and immunocytochemistry were used to detect MMP-1 and TIMP-1 expression in the cells. Results: Stronger expression of IL-1 (3 was found in orthodontic root resorption tissues of the dogs than in the normal tissues. The suitable dosage of IL-1(3 increased MMP-1 ex-pression( P <0. 05 ) and inhibited TIMP-1 expression( P <0. 05 ). Conclusion: IL-1(3 participate in tissue reaction in the process of root resorption by regulating MMP-1 and TIMP-1 expression in hPDLCs.%目的:探讨白细胞介素1(IL- 1β) 与正畸牙根吸收的关系.方法:建立犬牙根吸收的动物模型,应用PCR半定量检测IL- 1β mRNA在根吸收组织与正常根周组织之间表达的差异性.体外培养人牙周膜细胞(hPDLCs),设立IL- 1β工作浓度梯度:0.01、0.1、1、10 ng/ml,0 ng/ml为空白对照组,时间梯度:6、12、24 h,分别运用PCR 和免疫细胞荧光化学方法检测IL- 1β作用下,hPDLCs中MMP- 1、TIMP- 1表达的变化.结果:犬牙根吸收组织较正常根周组织的IL- 1β表达增强.适当剂量的IL- 1β可刺激MMP- 1的表达增高(P<0.05),抑制TIMP- 1的表达(P<0.05).结论:IL- 1β参与牙根吸收过程中的组织反应,其对hPDLCs中MMP- 1表达的促进作用和对TIMP- 1表达的抑制作用可能是牙根吸收发生的机制之一.
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