目的:研究白细胞介素(IL)-1α和集落刺激因子(CSF)-1对体外培养大鼠牙囊细胞骨保护素(OPG)表达的影响.方法:组织块结合酶消化法体外培养原代牙囊细胞并传代.取50ng/ml IL-1α或CSF-1刺激第4代牙囊细胞,western blot检测第0、1、3、6、12hOPG表达.采用不同浓度(0、4、10、50、250ng/ml)的IL-1α或CSF-1孵育第4代牙囊细胞12h,western blot检测OPG表达.结果:western blot结果显示随着IL-1α刺激时间和浓度的增加,OPG条带逐渐加深.随着CSF 1刺激时间的增加,OPG条带变浅但高于对照组;各CSF-1浓度组OPG条带无明显变化趋势,但高于对照组.结论:IL-1α、CSF-1均可上调大鼠牙囊细胞内OPG蛋白水平.%Objective:To invesligale the effect of IL-1α;, CSF-1 on OPG expression in ral denial follicle cells in viLro. Methods, For the lime-dependence sludy ihe ral denial follicle cells were cultured wilh 50 ng/ml IL-lα; or CSF-1 for 0, 1, 3, 6 and 12 h respectively, for the dose-dependence sludy the cells were cultured wilh different concentrations of IL-lα;( 0, 4, 10, 50, 250 ng/ml) or CSF-1 ( 0, 4, 10, 50, 250 ng/ml) for 12 h respectively, then the OPG expression was assayed by Western blot. Results, The Western blot showed that after stimulating by IL-lα;, the expression of OPG increased in a time and dose-dependent manner. After stimulating by CSF-1 for different times or by different doses, the expression of OPG decreased but higher than those in the control group. Conclusion, IL-lα; and CSF-1 both may increase the expression of OPG in rat dental follicle cells.
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