Objective: To investigate the expression and relation of Cox-2 and Ki-67 in normal salivary gland( NSG), basal cell adenoma(BCA) and basal cell adenoearcinoma(BCC). Methods: PV-6001 was used to detect the expression of Cox-2 and Ki-67 in the samples of 11 NSG, 18 BCA and 9 BCC. SPSS 12.0 software was used to analyse the experimental data. Results: Label index(LI) of Cox-2 expression in NSG, BCA and BCC was 3.72 ±2.59,33.33 ± 19.87 and 36.58 ±24.58 respectively( NSG vs BCA or BCC ,P ＜ 0.01 ); IL of Ki-67 expression was 2.89 ±2.63,4.66 ±4.58 and 32.27 ±22.77 respectively ( NSG vs BCC, P ＜0.01, NSG vs BCA,P ＞ 0.05 ). The expression of Cox-2 and Ki-67 has no statistical significance in BCA constitutional pathological typing(P ＞ 0.05). The expression of Cox-2 showed statistical significance in BCA genetic locus(P ＜ 0.01 ) , but that of Ki-63 did not( P ＞ 0.05 ). The expression of Cox-2 and Ki-67 was negatively correlated in BCA ( rs = - 0.21 , P ＞ 0.05 ) and positively correlated in BCC ( rs = 0.94, P ＜ 0.01 ). Conclusion: Cox-2 and Ki-67 may participate the procedure of BCA and BCC origination and development. United detection of Cox-2 and Ki-67 is helpful in the diagnose and prognosis of BCA and BCC.%目的:研究环氧合酶-2(Cox-2)及Ki-67在涎腺正常组织(normal salivary gland,NSG)、基底细胞腺瘤(basal celladenoma,BCA)和基底细胞腺癌(basal cell adenocarcinoma,BCC)中的表达及相关性,探讨其表达程度及在恶变中的相互关系.方法:采用免疫组化(PV6001二步法)检测NSG 11例、BCA 18例、BCC 9例中Cox-2及Ki-67的表达情况;采用SPSS 12.0软件对实验数据进行分析.结果:Cox-2在NSG中表达与BCA、BCC的差异均有统计学意义(P<0.01);Ki-67在NSG与BCA表达差异无统计学意义(P>0.05),在NSG与BCC中的表达差异有统计学意义(P<0.01);Cox-2、Ki-67表达与BCA组织病理分型的差异无统计学意义(P>0.05);Cox-2在不同部位的BCA中表达差异有统计学意义(P<0.01);Ki-67在不同部位BCA中表达差异无统计学意义(P>0.05);Cox-2和Ki-67在BCA表达中呈负相关(P>0.05),rs=-0.21,而在BCC表达中呈正相关,rs=0.94,P<0.01.结论:Cox-2、Ki-67可能参与BCA、BCC的发生、发展过程;联合检测Cox-2和Ki-67有助于BCA、BCC的预后判断.
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