Objective; To investigate the effect of two different methods of carboplatin administration on the drug-residence of human tongue cancer Tca8113 cells in vitro. Methods: Tca8113 cells were randomly divided into a control group,six tested groups and a large dose shock group. Celts were exposed to the corresponding concentrations of carboplatin. The half inhibitory concentration (ICM ) of each group was detected by MTT assay. The ERCC1 mRNA expression was delected by RT-PCR. Results: The value of ICW and ER-CC1 mRNA expression were gradually increased when the cells were exposed to increasing dose of carboplatin, furthermore, they were positively correlated(r =0. 878, P<0.01). The values of IC50 and ERCC1 mRNA of large dose shock group were between lower dose groups and higher dose groups ( P < 0. 05). Conclusion: Gradually increasing dose of carboplatin may induce drug-resistance of Tca8113 cells.%目的:探讨2种不同卡铂用药方式对人舌鳞状细胞癌株Tea8113耐药性的影响.方法:将Tca8113细胞株随机设置为1个对照组、6个不同程度的用药组和1个大剂量冲击用药组,然后加入相应浓度卡铂体外作用.用MTT法检测每组细胞的IC50值(半数抑制浓度),RT-PCR法检测每组细胞ERCC1 mRNA的表达.结果:随着药物浓度和作用次数的增加,小剂量逐步耐药法诱导的各组细胞IC50值和ERCC1 mRNA表达量均呈逐步递增的趋势,且它们呈正相关(r=0.878,P＜0.01)；大剂量冲击用药组细胞IC50值和ERCC1 mRNA表达量界于小剂量逐步耐药法的较小剂量组和较大剂量组之间,且有统计学差异(P＜0.05).结论:小剂量逐步多次用药法易引起Tca8113对卡铂的耐药性.
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