Objective:To determine the effect of Arg-Gly-Asp(RGD)-containing peptide on the expression of osteocalcin mRNA expression in mouse calvarial osteoblasts.Method:Titanium samples were coated with pure golden and then immersed into Arg-Gly-Asp-Cys (RGDC) solution (2 mmol/L) for overnight.The materials coated with Arg-Gly-Asp-Cys (RDGC) or without treatment were used as controls.Mouse calvarial osteoblasts were isolated and seeded on the surfaces and harvested in 10,15,and 21 d respectively.Osteocalcin mRNA was detected by Northernblot hybridization.Results:Osteocalcin expresion was found in the cells on RGDC coated surface on day 15,while that was found in the cells in other groups at same level on day 21.Conclusion: RGD peptide can promote the osteocalcin expression in mouse osteoblasts.%目的：观察材料表面固定粘附肽RGD对小鼠成骨细胞骨钙素mRNA表达的影响。方法：采用自组装单层结构法将含RGD的粘附肽RGDC共价结合在试件表面，建立RDGC和空白对照组。接种小鼠原代成骨细胞，分别收集培养第10，15，21 天的细胞，提取总RNA，Northern印迹杂交法检测骨钙素mRNA的表达。结果：RGD组第15 天即开始出现明显的骨钙素基因表达，对照组的基因表达出现在第21 天。结论：材料表面固定RGD有促进骨钙素基因表达，影响小鼠成骨细胞分化的功能。
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