在不同宽频电磁脉冲辐照条件(场强E=110、297、873 kV/m, 每个场强下的脉冲个数N=5、10次)下周期性(2次/d)辐照雄性大鼠两周(共28次),辐照结束后分别于6 h、1、3、7、10 d取样检测大鼠的血清睾酮含量、睾丸组织中超氧化物歧化酶(Superoxidase dismutase, SOD)与丙二醛(Malondialdehyde, MDA)含量及生精细胞凋亡率、大鼠的精子顶体酶活性、精子存活率等生物指标,通过与相同辐照条件下的单次辐照结果进行对比,考察多次重复辐照对雄性大鼠生殖系统的累积损伤效应及随后的修复情况.结果表明,多次重复辐照对雄性大鼠生殖系统具有累积损伤作用,其累积效果与单次辐照剂量有关,场强越高,脉冲个数越多,累积损伤效应越明显.与单次辐照损伤类似,累积损伤可随照后时间的延长得到一定程度的修复.%The present work focused on the cumulative damage caused by periodically repeated wide-band electromagnetic pulses. The exposure protocol was as follows: electric field intensity E=110 kV/m, 297 kV/m, and 873 kV/m; pulse number N=5, and 100; exposure was repeated 2 times per day, for 2 weeks. 6 h, 1 d, 3 d, 7 d, and 10 d after exposure, the testosterone concentration in blood plasma, superoxide dismutase (SOD) and malondialdehyde (MDA) in the testis, and acrosin activity of sperms were determined by ELISA. The apoptosis rate of spermatogenic cells in the testis was studied by TUNEL, and the survival rate of sperms in the epididymis by CASA. The results showed that compared with single exposure, 28 periodic exposures exhibited a cumulative damaging effect on the reproductive system of male rats. The cumulative effect increased significantly with increasing E and N. Similar to single exposure, the damage caused by repetitive exposure was gradually recovered to a certain degree with prolongation of exposure time.
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