用 X-射线照射不同浓度羧基荧光素-寡核苷酸-羧基四甲基罗丹明(Carboxyfluorescein-Oligonucleotide- carboxytetramethylrhodamine, FAM-DNA-TAMRA)水溶液,测量溶液发出的荧光强度,研究吸收剂量与荧光强度之间的关系.结果表明,对于含不同浓度FAM-DNA-TAMRA荧光探针的水溶液,在0?20 Gy剂量范围内,荧光强度与吸收剂量之间的线性关系很好,并且荧光稳定性良好,但存在荧光本底比较高,影响测量吸收剂量灵敏度的问题.研究结果提示,FAM-DNA-TAMRA荧光探针可以用于测量水溶液的吸收剂量.%To explore the relationship between absorbed dose and fluorescence intensity, FAM-DNA-TAMRA (Carboxyfluorescein-oligonucleotide-carboxytetramethylrhodamine) aqueous solution of different concentrations combined with X-ray irradiation was used to measure the fluorescence intensity. The results showed that the linear relation between fluorescence intensity and absorbed dose was good for different-concentration FAM-DNA-TAMRA aqueous solution containing fluorescent probe in the dose range of 0?20 Gy. The fluorescence had good stability, but the fluorescence background affected the sensitivity of absorbed dose greatly. The results suggest that the FAM-DNA-TAMRA fluorescent probe can be used to measure the absorbed dose of aqueous solution.
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